This utility makes it easy to identify what are the properties of a read based on its SAM flag value, or conversely, to find what the SAM Flag value would be for a given combination of properties.

下面是根据一个真实的bam文件统计的flag值和该值的总计数。 统计的代码为： 由于基本flag排列组合后得到的flag很多，这里展示有代表性的一部分，可以发现 83，163，99，147 出现的频 …

SAM stands for Sequence Alignment/Map format. It is a TAB-delimited text format consisting of a header section, which is optional, and an alignment section. If present, the header must be …

Mar 6, 2014 · BAM flags may not be intuitively understood by some. Here I write a post trying to explain how BAM flags work.

BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments. [5] . The goal of indexing is to …

The second column in a SAM/BAM file is the flag column; use the flags subcommand to understand specific flags. They may seem confusing at first but the encoding allows details …

Nov 20, 2013 · As we discussed earlier, the FLAG field in the BAM format encodes several key pieces of information regarding how an alignment aligned to the reference genome. We can …

This document is a companion to the Sequence Alignment/Map Format Specification that defines the SAM and BAM formats, and to the CRAM Format Specification that defines the CRAM …

Jun 25, 2024 · SAM, BAM and CRAM are all different forms of the original SAM format that was defined for holding aligned (or more properly, mapped) high-throughput sequencing data. SAM …

BAM stands for Binary Alignment Map, and is the compressed, binary version of a SAM file. This compression helps reduce the file size significantly. Most of the tools which operate on SAM …