Insert a BALF catheter into the mouse trachea and repeatedly inject 0.5 ml of ice cold PBS/5% FBS (from the “input” syringe filled with 3 ml of PBS/5% FBS) and then aspirate BALF from the inflated lungs into the initially unfilled “output” syringe via a 3-way stop cock.

Bronchoalveolar Lavage (BAL) is an experimental procedure that is used to examine the cellular and acellular content of the lung lumen ex vivo to gain insight into an ongoing disease state. Here, a simple and efficient method is described to perform BAL on murine lungs without the need of special tools or equipment.

Bronchoalveolar lavage (BAL) is a simple and typical method commonly performed to diagnose pulmonary diseases including lung cancer (Daubeuf and Frossard, 2012). It is used to sample pulmonary components to determine the protein composition, immune cells and …

May 1, 2004 · Cells were purified from BALF under sterile conditions using a FACS DIVA flow cytometric sorter (Becton Dickinson). BALF cells were stained as described above and different cell types were recognized based on scatter characteristics and expression of specific markers.

Feb 22, 2007 · The broncho-alveolar lavage fluid (BALF) is taken by injecting physiological solution through the tracheal tube into the murine airways and carefully regained by winding up the connected...

May 12, 2020 · Here, we performed single-cell RNA sequencing (scRNA-seq) on BALF cells from three patients with moderate COVID-19 (M1–M3), six patients with severe/critical infection (S1–S6), three healthy...

May 4, 2017 · Bronchoalveolar Lavage (BAL) is an experimental procedure that is used to examine the cellular and acellular content of the lung lumen ex vivo to gain insight into an ongoing disease state. Here, a simple and efficient method is described to perform BAL on murine lungs without the need of special tools or equipment.

Oct 27, 2023 · Anti-VEGF treatment increased the levels of BALF protein (Fig. 5D), BALF cells (Fig. 5E), and neutrophils (Fig. 5F) in WT mice, which were similar to those in IgG- or anti-VEGF-treated NOX4 KO mice (Fig. 5D-F).

Mar 15, 2008 · Bronchoalveolar lavage fluid (BALF) contains proteins derived from various pulmonary cell types, secretions and blood.

Bronchoalveolar lavage (BAL) is a technique used to collect the contents of the airways. The fluid recovered, called BAL fluid (BALF), serves as a dynamic tool to identify various disease pathologies ranging from asthma to infectious diseases to cancer in the lungs.