Sep 14, 2007 · It's the resistance to flow - the pushing back... It's called backpressure because it's established by the column/lines and depends on the fluid viscosity, flow rate, temperature etc., …

Dec 5, 2006 · When the pressure exceeds the back pressure exerted by the water, bubbles start passing through the water. This is known as a back pressure regulator. In LC and GC, the …

Feb 2, 2008 · This back pressure controls the flow into the column (ignoring the effects of the temperature in the GC oven causing changes in resistance to flow in the column). Lets pretend …

Apr 1, 2014 · If you don't have the p/n, there should still be a label clipped to the tubing advising of the pressure @ x flow rate. Based on your current pressure of 1400 psi, coupling the two red …

Apr 9, 2015 · In my first attempt I either crushed the particles in packing or online with too high of a pressure. It ended up running like size exclusion with the same profile regardless of gradient. …

Nov 26, 2019 · If you have one available, try installing it in place of the Max-Light cartridge and run your gradient 8 times. Depending on the flow cell you select, the cell volume may be larger …

May 24, 2008 · I classically use 250x3mm, 5µm C18 columns with CH3CN / water 5:95 at 0.6 ml/min. Back pressure obtained is 2700 PSI. It is usually said that the resolution can be …

Jul 30, 2012 · I have an Agilent 6890N and from a month ago I have this message" Shutdown 5 - back inlet pressure shutdown" I've checked for leaks, I cleaned the inlet tubing, I replaced the …

Jul 11, 2018 · This is basic step-wise troubleshooting. Plain and simple, if after removing the HPLC column and replacing it with a zero dead volume fitting AND running the same mobile …

Sep 6, 2024 · I am trying to troubleshoot Back Inlet Pressure Shutdown on my Agilent 7890B. I removed the septa and do not see a liner. We purchased some liners online but it appears …