The bromodomain and extra-terminal motif (BET) protein BRD4 binds to acetylated histones at enhancers and promoters via its bromodomains (BDs) to regulate transcriptional elongation.

Aug 3, 2018 · BRD4-eRNA interactions increased BRD4 binding to acetylated histones in vitro and augmented BRD4 enhancer recruitment and transcriptional cofactor activities. Our results suggest a...

BRD4 helps to assemble a functional Pol II elongation complex and recruits 3’-end RNA processing factors (CPSF, CstF) during a general 5’-elongation control point to allow productive elongation and proper RNA processing at the 3’-end of genes.

Dec 20, 2017 · Using two conditional knockout mouse strains and derived cells, we demonstrate that Brd4 controls cell identity gene induction and is essential for adipogenesis and myogenesis. Brd4...

Dec 22, 2023 · BRD4 recruits the positive transcription elongation factor b (P-TEFb) and Mediator, leading to RNA Polymerase II (RNA Pol II) activation and binding to enhancer- and super-enhancer-associated...

Dec 14, 2012 · Transcriptional elongation by RNA polymerase II (Pol II) is regulated by positive transcription elongation factor b (P-TEFb) in association with bromodomain-containing protein 4 (BRD4). We used genome-wide chromatin immunoprecipitation sequencing in primary human CD4+ T cells to reveal that BRD4 co- …

Dec 12, 2012 · Transcriptional elongation by RNA polymerase II (Pol II) is regulated by positive transcription elongation factor b (P-TEFb) in association with bromodomain-containing protein 4 (BRD4).

Mar 25, 2025 · The BioPs most highly affected by SMARCA4-dependent BRD4 peaks were post-transcriptional regulatory factors (kinases), cell adhesion, and RNA metabolism (Fig. 7D). Illustration of the differences in RSV-induced BRD4 peak distributions in WT versus KD cells across chromosomes are in Supplementary Fig. S5 .

We show that BRD4 occupies widespread genomic regions in mouse cells and directly stimulates elongation of both protein-coding transcripts and noncoding enhancer RNAs (eRNAs), in a manner dependent on bromodomain function.

Brd4 association with P-TEFb represents the active complex without the inhibitory components HEXIM1/2 and 7SK small nuclear RNA. How Brd4 transits from chromatin targeting to transcriptional regulation in response to environmental cues is a crucial issue for our understanding of epigenetic control in eukaryotic transcription.