Mar 1, 2025 · We found that targeted inhibition of bromodomain-containing protein 4 (BRD4) by siRNA or its inhibitor (JQ1) significantly decreases mRNA m 6 A levels and suppresses the malignancy of breast cancer (BC) cells via increased expression of …

These findings provide insight into the role of BRD4 in epigenetic regulation of gene pathways that promote the metastatic phenotype of cancer cells and suggest that therapies targeting BRD4 may represent a strategy to prevent cancer metastasis.

Apr 9, 2025 · We show that these focal deletions occur in the context of cyclin E1 amplification in breast, ovarian, and endometrial cancers, and serve to disrupt BRD4 regulatory regions and gene expression across isoforms.

A knockdown of BRD4 allows the retention of MTOR activity during starvation and significantly undermines starvation-induced cell death. However, BRD4 repression only contributes to stimulus-dependent autophagy and aggrephagy, and …

Epigenetic factor Brd4 has emerged as a key regulator of cancer cell proliferation. Targeted inhibition of Brd4 suppresses growth and induces apoptosis of various cancer cells.

Aug 17, 2023 · Although BRD4-PTEFb can associate with chromatin through acetyl recognition, our results indicate that a distinct, active BRD4-PTEFb population functions to regulate transcription independently of bromodomain-mediated chromatin association. These findings may enable more effective pharmaceutical modulation of BRD4-PTEFb activity.

Mar 25, 2025 · BRD4 CUT&RUN tags are displayed along with NFR ATAC-seq from mock-infected or RSV infected WT and SMARCA4 KD cells. Zoomed image is shown at right. Note the increase in super enhancer peaks in RSV infected versus mock infected WT cells (arrowhead) and reduction in the SMARCA4 KD cells (*).

BRD4 has been used as a therapeutic target for various inhibitors that displace it from the chromatin (4, 8–16). However, BRD4 upstream regulation by posttranslational modifications is poorly understood. Here, we identify BRD4 as a novel substrate for methylation by SETD6.

Jun 25, 2021 · BRD4 inhibitors (or the knockdown of BRD4 expression) significantly inhibit the malignant behavior of glioma cells or GSCs, resulting in effective therapeutic targeting of these tumors. Thus, BRD4 inhibitors can significantly delay …

Mar 6, 2024 · To explore how SASP is transcriptionally regulated in beta cells, we used the NOD beta cell line NIT-1 to model beta cell SASP and identified binding partners of BET protein Brd4 and explored the role of the cyclin-dependent kinase inhibitor p21.