May 9, 2023 · In this study, we employed a BRD4-targeting PROTAC molecule to regulate the super-enhancer (SE) condensate and monitored the changes of SE condensate under PROTAC treatment using live-cell...

Mar 16, 2020 · Here, we report that, in human cancer cells, BRD4S forms nuclear puncta that possess liquid-like properties and that colocalize with BRD4L, MED1 and sites of histone H3 lysine 27 acetylation....

Jul 27, 2018 · Here we demonstrate that the SE-enriched transcriptional coactivators BRD4 and MED1 form nuclear puncta at SEs that exhibit properties of liquid-like condensates and are disrupted by chemicals that perturb condensates.

Jan 21, 2022 · Our transcriptional analyses indicate that the PCG-induced inhibition of BRD4 LLPS was as effective in suppressing transcription of MYC and other BRD4-dependent genes as the JQ1-mediated dissociation of BRD4 from the chromatin templates.

Nov 9, 2021 · Phosphorylation of BRD4 therefore promotes dimerization and inhibits interaction with DNA and formation of LLPS, while being necessary for active gene transcription.

The addition of BRD4, which binds acetylated histone tails, restores LLPS of acetylated chromatin. The resulting condensates cannot mix with unmodified chromatin condensates, yielding two distinct phases, similar to distinct chromatin domains in the nucleus.

We achieve this advance by driving optogenetic TFs to undergo liquid-liquid phase separation (LLPS) and to form TF droplets around their target promoters. Eukaryotic cells are highly compartmentalized into specific organelles, which are either membrane enclosed or …

(C) CTCF-mediated chromatin looping provides an architectural framework for the local enrichment of various transcriptional machineries (e.g., Pol II, MED1 and BRD4) and drives the formation of transcriptional condensates via LLPS. Depletion of …

Dec 1, 2020 · FUS, BRD4 and TAF15 proteins can undergo LLPS induced by the CasDrop system. As the droplets grow, chromatin in the region where the droplets are located is repelled from the area [85]. In addition, the study also found that BRD4 can be induced to form droplets in low-density regions of chromatin.

Feb 11, 2022 · Recent evidence also implicates the BRD4 short isoform (BRD4S) in driving BRD4 LLPS and influencing BRD4 function in gene transcription as larger BRD4 puncta size was observed to correlate...