Dec 20, 2017 · Using two conditional knockout mouse strains and derived cells, we demonstrate that Brd4 controls cell identity gene induction and is essential for adipogenesis and myogenesis. Brd4...

Aug 17, 2023 · HiS-NET-seq uncovers new transcribed enhancers in human cells. A multi-omics analysis shows that genome-wide enhancer transcription depends on the BET family protein BRD4. Specifically, BRD4...

Nov 21, 2024 · JNK-mediated BRD4 functional switching induces CD8 expression in thymocytes and epithelial-to-mesenchymal transition (EMT) in prostate cancer cells. These findings elucidate the mechanism by which BRD4 transitions from a chromatin regulator to a …

Aug 17, 2023 · BRD4 depletion led to a dramatic reduction in the CCNT1 peaks at promoters genome-wide, indicating that CCNT1 recruitment to chromatin relies on BRD4 (Figures 5A and S5A) but not vice versa, as CDK9 depletion leads to …

We found that BRD4 interacts with the LSD1/NuRD complex and colocalizes with this repressive complex on super-enhancers and that decommissioning BRD4-directed super-enhancer organization of transcription repression programs inflicts widespread drug …

Dec 12, 2012 · To establish whether BRD4 regulates transcription elongation in a genome-wide manner, we performed ChIP followed by DNA sequencing in primary human CD4+ T cells to identify 33,544 BRD4 binding sites (peaks).

Nov 21, 2024 · The key evidence demonstrates JNK activation to release wild-type, but not 3A mutated (S1153A/T1222A/T1248A), BRD4 from the chromatin. With chromatin immunoprecipitation (ChIP)-seq, this detachment is shown to occur genome wide.

Aug 14, 2020 · Whole genome BRD4 and γH2AX ChIP-Seq with R-loop IP qPCR reveals that BRD4 inhibition leads to accumulation of R-loops and DNA damage at a subset of known BDR4, JMJD6, and CHD4 co-regulated...

To address changes in BRD4 occupancy during OIS, we next performed ChIP-Seq profiling for BRD4 in proliferating, quiescent and senescent IMR90 cells.

Jan 31, 2023 · In this study, we report that BRD4 works with Polycomb repressive complex 2 (PRC2) to repress transcriptional expression of the T‐helper 2 (Th2)‐negative regulators Foxp3 and E3‐ubiqutin ligase Fbxw7 during lineage‐specific differentiation of Th2 cells from mouse primary naïve CD4 + T cells.