We developed a novel virus-like particle (VLP) vaccine composed of bovine papillomavirus L1 protein engineered to display surface docking sites.

Mar 14, 2017 · Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity.

Jun 7, 2023 · The full-length open reading frame (ORF) of bovine papillomavirus (BPV) type 1 L1 (GenBank: BBB04658.1) preceded by a Kozak consensus sequence, with codons modified for efficient expression in insect cells, was constructed by PCR-based gene synthesis.

Apr 10, 2025 · Objective: The current study aims to characterize the genetic variation in the L1 gene of the BPV, explore in silico the protein structure, predict epitopes, and evaluate the impact of mutation on the epitope conservancy.

BPV-1 L1 VLPs may constitute a safe and highly immunogenic vaccine candidate for protection of horses against BPV-1/-2-induced disease. Three groups of 4 horses each received 50, 100 or 150 μg of BPV-1 L1 VLPs, respectively, on Days 0, 28 and …

L2 possesses a second independent L1 interaction domain located between residues 129 and 246. This interaction was detected in vivo, but not in vitro. Both L1 interaction domains are necessary for L2 to efficiently encapsidate the BPV1 genome in BPHE-1 cells.

Sep 1, 1996 · To identify areas within the bovine papillomavirus type-1 L1 (BPV L1) protein that are important for virus assembly, we constructed a set of 24 baculovirus recombinants expressing BPV L1 deletion mutants that span the entire L1 open reading frame.

Nov 4, 2013 · Here, we utilized a mucosal vaccine vector, bovine papillomavirus (BPV) virus-like particles (VLPs), as a platform to present HIV-1 neutralizing epitopes by inserting the extended 2F5 or 4E10 epitope or the MPER domain into D-E loop of BPV L1 respectively.

Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases.

Mar 14, 2017 · In this preliminary study, we aimed to integrate data about BPV-1 L1 gene cloning and expression from a bacterial system (E. coli). Afterward, we characterized the L1 recombinant protein and structure of VLPs regarding their biosafety and thermostability.