Escherichia coli BL21(DE3) is a commonly used protein production strain of the E. coli bacterium. This strain combines several features that allow for excessive expression of heterologous proteins. It is derived from the B lineage of E. coli. [1]

BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain. Ideal for routine T7 expression; Protease deficient B strain; No dry ice surcharge on competent cell shipments; Outgrowth medium included; Free of animal products

Thermo Scientific BL21 (DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

Jan 11, 2016 · BL21(DE3) is suitable for expression from a T7 or T7-lac promoter or promoters recognized by the E.coli RNA polymerase: e.g. lac, tac, trc, ParaBAD, PrhaBAD and also the T5 promoter.

The BL21 (DE3) E. coli strain is one of the most popular host strains to produce recombinant proteins. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of the lac UV5 promoter.

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and ...

E. coli BL21(DE3), a derivative of BL21, is probably the most widely used in high-level expression of recombinant proteins, and it harbors a prophage DE3 derived from a bacteriophage λ, which carries the T7 RNA polymerase gene under the control of the lacUV5 promoter.

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system. Components •11 rxn or 22 rxnBL21(DE3) Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid. Warning.

Oct 28, 2024 · The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase.

BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site.