Oct 14, 2018 · Endothelial dysfunction is oxidative stress driven by low levels of BH4 at the Nitric Oxide Synthase enzyme (NOS), called NOS-uncoupling; where NOS-Uncoupling is a …

Sepiapterin effectively enhanced BH4 levels in hyperoxia-exposed retinas and brains, elevated NOS activity, and reduced NT-modified protein, leading to reversal of the exacerbated …

Jun 20, 2003 · Tetrahydrobiopterin (BH4) serves as a critical co-factor for the endothelial nitric-oxide synthase (eNOS). A deficiency of BH4 results in eNOS uncoupling, which is associated …

The hypothesis of NOS/BH4 stoichiometry loss was demonstrated by eNOS suppression in metastatic melanoma cells. Decreased Nos3 expression abrogated the increase in O 2•− and …

In this review, we discuss the synthesis of BH4, its molecular mechanisms regulating eNOS coupling, the pathophysiologic roles of decreased BH4 bioavailability in cardiovascular …

eNOS-BH4 Interaction Stoichiometry —The stably transfected doxycycline-responsive GCH/eNOS cells enable manipulation of the intracellular eNOS:BH4 ratio and, hence, …

Nitric oxide, generated by the nitric oxide synthase (NOS) enzymes, plays pivotal roles in cardiovascular homeostasis and in the pathogenesis of cardiovascular disease. The NOS …

Jan 1, 2017 · Results: Sepiapterin effectively enhanced BH4 levels in hyperoxia-exposed retinas and brains, elevated NOS activity, and reduced NT-modified protein, leading to reversal of the …

Mar 9, 2023 · Here, we discuss the main mechanisms of eNOS uncoupling, including oxidative depletion of the critical eNOS cofactor BH 4, deficiency of eNOS substrate L-Arg, or …

Dec 4, 2003 · The pteridine cofactor tetrahydrobiopterin (BH4) has emerged as a critical determinant of eNOS activity: when BH4 availability is limiting, eNOS no longer produces NO …