The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.

The Thermo ScientificTM PierceTM BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.

The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein ...

BCA Protein Assay with Dilution-Free BSA Protein Standards – green-to-purple (A562 nm), 2-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard in 30 minutes at 37°C.

Nov 1, 2020 · Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility.

Bicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. BCA rely on the formation of a Cu 2 + –protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + (Smith et al., 1985).

The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu 2+ ] is reduced to copper (I) [Cu 1+ ] in the presence of peptides in alkaline solutions.

The Bicinchoninic Acid (BCA) protein assay is a reliable and sensitive method for the quantification of total protein in a sample. It is more sensitive than the Bradford assay and can detect protein concentrations as low as 0.5 μg/mL.

The BCA protein assay is based on a biuretreaction, which is the reduction of Cu 2+ toCu + by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. Bicinchoninic acidis a chromogenic reagent that chelates thereduced copper, producing a purple complexwith strong absorbance at 562 nm (Smith1985, Wiechelman 1988).

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.