Pierce™ BCA Protein Assay Kits - Thermo Fisher Scientific
A high-precision, detergent-compatible protein assay for determination of protein concentration. The assay is based on the reduction of Cu 2+ to Cu 1+ by protein and the detection of the cuprous cation by BCA.
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Explore our BCA assay kits, that provide high-precision, detergent compatible protein quantification determination.
Bicinchoninic acid assay - Wikipedia
The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. The total protein concentration is exhi…
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BCA Assay and Lowry Assays | Thermo Fisher Scientific - US
- The first step is the chelation of copper with protein in an alkaline …
- In the second step, BCA reagent, a highly sensitive and selective colorimetric …
- The Pierce Dilution-Free Rapid Gold BCA Assay reduces setup time by up to …
- Pierce Dilution-Free Rapid Gold BCA Protein Assay generates a linear …
- Protein quantification results of the Pierce Dilution-Free Rapid Gold BCA …
Protein Quantification: BCA Assay – QB3 Berkeley
Learn how to measure the total protein concentration of an unknown sample using the bicinchoninic acid (BCA) assay. Follow the procedure, construct a standard curve, and use the …
Protein quantification by bicinchoninic acid (BCA) assay follows ...
Nov 1, 2020 · Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility.
- Author: Javiera Cortés-Ríos, Ana María Zárate, Juan David Figueroa, Joaquín Medina, Eduardo Fuentes-Lemus, M...
- Publish Year: 2020
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BCA Principle: - Assay-Protocol
Learn how to use the BCA assay, a colorimetric method for protein quantitation, based on the reduction of Cu +2 by protein and the detection of Cu +1 by BCA. Find the working solution …
Bicinchoninic Acid Assay - an overview | ScienceDirect Topics
Learn about the BCA assay, a protein quantification technique that uses a weak acid to produce a purple complex with copper ions. Find chapters and articles on the principle, procedure, …
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Determination of Protein by Bicinchoninic Acid Assay
Learn how to use the BCA method, based on the reduction of Cu 2+ to Cu + by proteins, to quantify proteins in aqueous samples. Follow the experimental steps, reagents, instruments, …
Protocol for Bicinchoninic Acid (BCA) Protein Assay
Learn how to use the Bicinchoninic Acid (BCA) assay to quantify total protein in various sample types. The protocol includes materials, reagents, procedure, tips, and a standard curve example.
Protein Determination by the Bicinchoninic Acid (BCA) Method
Learn how to use the Bicinchoninic Acid (BCA) method to quantify protein concentration in a solution. Follow the standard protocol, reagent preparation, and data analysis steps with …