Dec 9, 2022 · As a proof of concept, we targeted BACE1, phospho-tau, and HER2 to assess the ability of TEMs to deliver an antibody therapeutic to the brain with measurable activity. Our studies demonstrated that the TEM can effectively cross the BBB in vivo and mediate NCP.

Aug 4, 2016 · In this study, we examined both the changes in BBB permeability in STZ-induced diabetic CD-1 mice and morphologic changes in the BBB and the neurovascular unit. We visualized these changes using transmission electron microscopy (TEM).

May 25, 2023 · Blood–brain barrier (BBB) is a natural protective membrane that prevents central nervous system (CNS) from toxins and pathogens in blood.

Transcytosis-enabling modules (TEMs) using human transferrin receptor or CD98 binders and an engineered Fc cross the blood-brain barrier and induce phagocytosis of phospho-tau aggregates or HER2+ tumor cells without reticulocyte depletion or release of neurotoxic cytokines.

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Sep 1, 2014 · To examine the role of PECAM-1 and CD99 for monocyte TEM at the BBB in vitro, we performed quantitative endpoint assays in the presence of blocking mAb against PECAM-1, CD99, or nonblocking control mAb against VE-cadherin. TEM was unaffected in control conditions and proceeded to a maximal 90%.

Sep 25, 2023 · In neuroinflammation, peripheral immune cells cross an inflamed BBB through a multi-step cascade of interconnected events leading to transendothelial migration (TEM). TEM is a dynamic and sequential process that involves an initial tether of leukocytes onto the BBB endothelial cell surface.

Jan 12, 2023 · These results revealed that Mfsd2a/Cav-1 mediated transcytosis is a key pathway governing BBB BMECs permeability induced by P. gingivalis, which may contribute to P. gingivalis /virulence factors...

In this work, MIMETAS scientists developed a human BBB-on-a-chip to study neuroinflammation and transendothelial migration (TEM). This model comprises of primary human brain microvascular endothelial cells (HBMECs) cultivated under bidirectional perfusion.

Nov 1, 2021 · In this review paper, we (1) compared the current BBB experimental models, (2) briefly summarized their fabrication considerations, (3) discussed the detection techniques used in such models, and (4) provided suggestions to incorporate …