avGFP is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 1992, derived from Aequorea victoria. It has low acid sensitivity.

In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.

May 11, 2016 · Here we visualize an extensive region of the local fitness landscape of the green fluorescent protein from Aequorea victoria (avGFP) by measuring the native function (fluorescence) of tens of...

Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP).

Feb 1, 2017 · As an alternative to avGFP for live cell fluorescence imaging, UnaG has the inherent advantages of a smaller gene size (which may facilitate packaging in viral vectors with limited DNA capacity), and the ability to develop fluorescence in anaerobic conditions when bilirubin is supplied from serum.

FPbase is a free and open-source, community-editable database for fluorescent proteins and their properties.

Jul 30, 2024 · This study aims to utilize Transformer models and large language models (such as GPT and Claude) to predict the brightness of Aequorea victoria green fluorescent protein (avGFP) and design mutants with higher brightness.

Jun 2, 2010 · A variety of intrinsically fluorescent proteins (FP) from the superfamily of Aequorea victoria green FP (avGFP)-like proteins have proven to be popular and powerful tools for live cell fluorescence imaging applications.

avGFP (wt GFP) excitation: 395 nm and emission: 509 nm. avGFP is a 26.9 kDa fluorescent protein derived from Aequorea victoria. Learn more about avGFP.

Jan 1, 2010 · In AvGFP, the continuous proton wire connecting the chromophore phenol to a prepared Glu222 acceptor is likely an accelerating factor of ESPT. This role is suggested by time-resolved vibrational spectroscopy, showing that a glutamate, most likely Glu222, becomes protonated upon neutral chromophore excitation, a reaction that takes place on ...