The auxin-inducible degron 2 technology provides sharp degradation …
Nov 11, 2020 · AID2, which employs an OsTIR1 (F74G) mutant and a ligand, 5-Ph-IAA, shows no detectable leaky degradation, requires a 670-times lower ligand concentration, and achieves …
The auxin-inducible degradation (AID) system enables versatile ...
To address this, we have adapted the auxin-inducible degradation (AID) system discovered in plants to enable conditional protein depletion in C. elegans. We report that expression of a …
An efficient auxin-inducible degron system with low basal degradation …
Aug 26, 2019 · The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with …
The auxin-inducible degradation (AID) system enables versatile
Dec 15, 2015 · To address this, we have adapted the auxin-inducible degradation (AID) system discovered in plants to enable conditional protein depletion in C. elegans. We report that …
Protein degradation in auxin response - Oxford Academic
Apr 23, 2024 · Key switches in molecular signaling events triggered by auxin, and their proper homeostasis, require rapid and well-timed degradation of specific auxin response proteins …
ARF degradation defines a deeply conserved step in auxin response
1 day ago · This identifies ARF degradation as a key and ancestral property of the auxin response. In land plants, the signalling molecule auxin profoundly controls growth and …
Methods for rapid protein depletion in C. elegans using auxin …
One such method that is proving to be exceptionally powerful is auxin-inducible degradation (AID). In order to administer AID in C. elegans, a 44 amino acid degron tag is added to the protein of …
An improved auxin-inducible degron system preserves native …
We found that coexpressing the PB1 domain of ARF improved the robustness of the AID system by rescuing auxin-independent degradation and increasing the rate of auxin-induced …
A conserved ARF–DNA interface underlies auxin-triggered ... - PNAS
Apr 1, 2025 · The plant signaling molecule auxin coordinates various developmental programs (1–3).This process is mainly governed by the nuclear auxin pathway (NAP), which is …
A KNOTTED1-LIKE HOMEOBOX PROTEIN1–interacting ... - Science …
Mar 19, 2025 · The local auxin response in AZ cells is mediated by auxin signaling. The auxin-promoted degradation of Aux/IAAs (AUXIN/indole-3-acetic acids) releases the repression …