The bacteriophage phiC31 integrase is a recombinase that mediates the sequence-specific recombination between two attachment sites, attB and attP.

Recombination of attP and attB sites creates attL and attR sites. Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA …

Feb 27, 2007 · We generated a collection of lines with precisely mapped attP sites that allow the insertion of transgenes into many different predetermined intergenic locations throughout the …

Nov 1, 2024 · In addition to promoting attR x attL reaction, the RDF inhibits attP x attB recombination. This feature makes the directionality of integrase reactions programmable, …

Jul 14, 2017 · Both HK and ΦC31 systems comprise attB / attP attachment sites that serve as points of recombination, and the recombinases that catalyze recombination. In each family, …

Here we show that mutations in attB can block the integration reaction at different stages. Mutations at positions distal to the crossover site inhibit recombination by destabilizing the …

To integrate a transgene, Int recombines attP (in the landing site) with the corresponding attB sequence on the plasmid (Figure 1A). Following integration, the transgene is flanked by the …

Jan 11, 2021 · These events occur by recombination at specific attachment sequences on the phage DNA and on the bacterial chromosome called attP (from the phage) and attB (from the …

Jan 4, 2018 · Here, we isolated two novel pseudo attP sites in the pig genome using ФC31 integrase and characterized them as safe harbors for site-specific transgene integration and …

Which attP stock should I chose for my microinjection? Ideally, the expression of a transgene inserted at an attP site should not be influenced by local enhancers or chromatin effects. …