An overview of Thermo Fisher Scientific's Gateway Cloning technique and how to simulate the Gateway reaction in SnapGene.

You can use Gateway cloning to insert multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one …

If you want to transfer your attB-flanked PCR product directly into an expression clone, you can easily combine the BP and LR reactions using the following protocol.

耗时短：Gateway克隆系统可在一天内生成expressin载体，而传统的酶切连接克隆需要2天以上的时间。 Gateway克隆也能在同一管中完成BP和LR克隆反应，加速了attB-PCR产物直接克隆 …

This manual provides an overview of the Gateway® Technology and provides instructions and guidelines to: Design attB PCR primers and amplify your sequence of interest.

Access tips and tricks to meet your Gateway® and Seamless Cloning needs covering BP/LR reactions, Seamless Cloning, High-Order Genetic Assembly and Type IIs Assembly.

The BP clonase enzymes recombine the attB and attP sites, replacing the Gateway cassette with the amplified insert, which is now flanked by attL or attR sites depending on the configuration …

Gateway attB1 and attB2 sequences are added to the 5' and 3' end of a gene fragment, respectively, using gene-specific PCR primers and PCR amplification. The PCR amplification …

Learn how to simulate Gateway® cloning in Geneious Prime, including how to automatically design attB-linked oligonucleotide primers, simulate BP and LR reactions.

Jan 11, 2021 · List of orthogonal attB sequences used in the Gateway recombination system. Changes in the core region (O) with respect to the wild-type attB sequence are indicated by …