Atg8-PE protein-based in vitro biochemical approaches to
Recently, several biochemical approaches have been successfully developed to produce the lipidated protein Atg8-PE or its mimics in vitro, including enzyme-mediated reconstitution …
ATG8 - Wikipedia
Autophagy-related protein 8 (Atg8) is a ubiquitin-like protein required for the formation of autophagosomal membranes. The transient conjugation of Atg8 to the autophagosomal …
Atg8–PE protein-based in vitro biochemical approaches to …
Recently, several biochemical approaches have been successfully developed to produce the lipidated protein Atg8–PE or its mimics in vitro, including enzyme-mediated reconstitution …
A role for Atg8-PE deconjugation in autophagosome biogenesis
May 1, 2012 · Approximately one-fourth of all of the characterized Atg proteins that participate in autophagosome biogenesis affect Atg8, regulating its conjugation to …
Membrane perturbation by lipidated Atg8 underlies ... - Nature
Jul 8, 2021 · Atg8–PE is produced onto lipid bilayer nanodiscs incorporating 70 mol% POPC and 30 mol% POPE via a lipidation reaction mixture containing Atg7, Atg3, Atg12–Atg5–Atg16N, …
Atg8, a Ubiquitin-like Protein Required for ... - ScienceDirect
Jul 13, 2007 · Immunoelectron microscopy revealed that Atg8, probably as a PE-conjugated form (Atg8-PE), is predominantly localized on the isolation membranes rather than on the complete …
A role for Atg8–PE deconjugation in autophagosome biogenesis
Approximately one-fourth of all of the characterized Atg proteins that participate in autophagosome biogenesis affect Atg8, regulating its conjugation to …
Dual roles of Atg8−PE deconjugation by Atg4 in autophagy
Atg8 is a unique Ubl protein whose conjugation target is the lipid phosphatidylethanolamine (PE). Atg8 functions in the formation of double-membrane autophagosomes, a central step in the …
Activation and targeting of ATG8 protein lipidation
May 5, 2020 · ATG8 lipidation is a hallmark of autophagy where these proteins are involved in autophagosome formation, the fusion of autophagosomes with lysosomes and cargo selection. …
ATG8 lipidation and ATG8‐mediated autophagy in Arabidopsis …
Apr 16, 2010 · In this study, we developed a method to reliably detect the ATG8-PE adduct and demonstrate its absence in backgrounds containing previously reported or new mutant alleles …