Nov 4, 2011 · Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like protein (UBL) Atg8 …

Jan 8, 2018 · Here we report a thiol-dependent process that may account for impaired autophagy during aging. This is through direct oxidation of key autophagy-related (Atg) proteins Atg3 and …

Binding Features of ATG3 Interaction Between ATG3 and ATG7. ATG3 forms an E1–E2 complex with ATG7, which is unique compared with other protein-conjugation systems. An in vitro pull …

Nov 11, 2012 · Crystallographic and mutational analyses of yeast (Atg7–Atg3) 2 and (Atg7–Atg10) 2 complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. …

Jul 7, 2015 · Here, our NMR data precisely define the region of the FR of human ATG3 that interacts with ATG7 (RIA7) and demonstrate RIA7 partially overlaps with the E3-interacting …

Oxidation of ATG3 and ATG7 facilitates autophagy inhibition (Frudd et al, 2018). Endogenous ATG7 deletion prevents ATG8 lipidation, so ATG7 knockout (KO) models are commonly used …

Jun 23, 2021 · We found deleterious, recessive variants in human ATG7, a core autophagy-related gene encoding a protein that is indispensable to classical degradative autophagy. …

Here we report a thiol-dependent process that may account for impaired autophagy during aging. This is through direct oxidation of key autophagy-related (Atg) proteins Atg3 and Atg7. When …

Nov 4, 2011 · Here we report that the unique N-terminal domain of Atg7 (Atg7 NTD) recruits a unique “flexible region” from Atg3 (Atg3 FR). The structure of an Atg7 NTD -Atg3 FR complex …

The E1-like enzyme ATG7 is one of the core components that are directly involved in LC3 lipidation reaction. Here, we provide evidence showing that acetylation of ATG7 tightly controls …