Mar 12, 2018 · Ectodomain shedding of AREG requires ADAM17. To determine whether ADAM17 is required for AREG ectodomain shedding, we asked whether loss of ADAM17 in Rhbdf2 cub/cub keratinocytes alters AREG secretion with or without stimulation with 100 n m phorbol‐12‐myristate‐13‐acetate (PMA).

Among other things, ductal development requires the epidermal growth factor receptor (EGFR), its ligand amphiregulin (AREG), and the transmembrane metalloproteinase ADAM17, which can cleave and release AREG from the cell surface so that it may interact with its receptor.

Jun 6, 2024 · The I386W mutation in iRhom2 led to loss of ADAM17-dependent shedding of AREG (Figure 3B), loss of detectable mature ADAM17 (Figure 3C), and abolished physical interaction between iRhom2 and ADAM17 (Figure 3D).

Aug 8, 2016 · Injury drives upregulation of ADAM17 and of its substrates, in particular pro-AREG. Increased ADAM17 activity on the cell surface leads to enhanced release of soluble AREG and TNFα, which activate their respective receptors. In a positive feedback loop, AREG-mediated EGFR activation increases ADAM17 and AREG expression.

Mar 12, 2018 · In the present study, using a curly bare (Rhbdf2cub ) mouse model that shows loss-of-hair, enlarged sebaceous gland, and rapid cutaneous wound-healing phenotypes mediated by enhanced Areg mRNA and protein levels, we sought to identify the principal ectodomain sheddase of AREG.

Shedding of extracellular Pro-AREG domain can be initiated by TACE enzyme - a member of the disintegrin and metalloproteinase (ADAM) family also known as ADAM17. After the Pro-AREG cleavage at Lys187 is complete, secreted mature ligand behaves as an autocrine or paracrine factor on the adjacent cells with its consanguine receptor - epidermal ...

The above data demonstrate that the ADAM17–EGFR axis is an essential paracrine pathway whereby ADAM17 releases epithelial AREG, which then activates stromal EGFR, thus eliciting reciprocal responses that further orchestrate mammary epithelial development.

May 10, 2008 · Among other things, ductal development requires the epidermal growth factor receptor (EGFR), its ligand amphiregulin (AREG), and the transmembrane metalloproteinase ADAM17, which can cleave and release AREG from the cell surface so that it …

Jun 1, 2021 · ADAM17 is an important regulator of TNF-α pathway. Besides cleavage of TNF-α itself, which is necessary in order to bind and activate its receptor, ADAM17 also sheds TNF receptor 1 (TNF...

Aug 23, 2022 · We show that ADAM17 sheds heparin-binding EGF (HB-EGF) and amphiregulin (AREG) from the cancer cell surface, leading to EGFR activation in macrophages. Moreover, we demonstrate that ADAM17/HB-EGF signaling regulates the education of protumorigenic macrophages, the expression and secretion of chemokines, as …