... propose an APTES- (EDC/NHS) coating technique to combine APTES silanization with the modified EDC/NHS crosslinking as shown in Figure 3. Firstly, the silicon surface was treated with...

Surface functionalization and bioreceptor immobilization are critical processes in developing a highly sensitive and selective biosensor. The silanization process with 3-aminopropyltriethoxysilane (APTES) on oxide surfaces is frequently used for ...

May 18, 2021 · In this study, we proposed a new coating technique to avoid such problem by applying APTES silanization with 1-ethyl-3- (3-dimethyl aminopropyl)-carbodiimide (EDC)- N -hydroxysuccinimide (NHS) protein crosslink, denoted by the APTES- (EDC/NHS) method.

In the present study on APTES-functionalized bioanalytical platforms, various EDC-based antibody crosslinking chemistries are compared, where EDC binds initially to the carboxyl groups on the antibodies followed by the subsequent formation of amide bonds with the amino groups present on the surface.

Jul 13, 2012 · In the present study on APTES-functionalized bioanalytical platforms, various EDC-based antibody crosslinking chemistries are compared, where EDC binds initially to the carboxyl groups on the antibodies followed by the subsequent formation of amide bonds with the amino groups present on the surface.

Jan 2, 2022 · Hall et al. reported phenyltriethoxysilane (PTES) show compatibility with amino, thiol and allyl trialkoxysilane derivatives. Bifunctionalized organosilica MCM-41 was synthesized via premix TEOS, PTES with allyltrimethoxysilane, 3-aminopropyltriethoxysilane (APTES) or 3-mercaptopropyltriethoxysilane (MPTES), respectively [132].

The obtained average surface roughness of the anti-CRP surfaces shows the largest increase in case of the EDC coupling to the silanized surfaces using 96% ethanol as the solvent, starting from (0.51 ± 0.09) nm to (0.83 ± 0.09) nm and further to (2.0 ± 0.2) nm for the silanization parameters (C APTES = 1%, t = 10 min), (C APTES = 2%, t = 20 ...

In the conventional APTES-GA technique, we measured the increased thickness at four stages: (i) initial SiO2 layer due to Si surface oxidization of the sample, (ii) APTES layer, (iii) APTES-GA...

Mar 18, 2014 · An improved enzyme-linked immunosorbent (ELISA) assay using one-step antibody immobilization has been developed for the detection of human fetuin A (HFA), a specific biomarker for atherosclerosis...

Oct 1, 2024 · The sensor employs the covalent immobilization of uricase enzyme using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) crosslinking agents, ensuring the durable adherence of the enzyme onto the sensor probe.