Oct 23, 2013 · The team plans to differentiate the stem cells into other tissues, such as neurons, and compare how the cells from different species behave. They will also use the iPSC technology to investigate how chimpanzees might differ from people in susceptibility to cancer, genetic diseases and viral infection.

Feb 3, 2023 · In this Review, we describe how the sequencing of genomes from modern and archaic hominins, great apes and other primates is revealing human-specific genetic changes and how new molecular and...

In our bulk RNA-seq data of iPSC-derived PrSyn, CGA is the most highly expressed protein coding transcript of all, with over 30,000 FPKM (fragments per kilobase of transcript per million mapped reads). An additional trophoblast bona fide of our iPSC-derived PrSyn is the expression of CYP19A1 from its trophoblast-specific promoter (Figure S3B).

Mar 18, 2025 · In this study, we successfully reprogrammed skin fibroblasts from three small ape species using SRVs, and established five iPSC lines from one lar gibbon and two siamangs. Prior to this study, we also attempted to reprogram small ape cells using plasmid or SeV vectors.

Feb 4, 2025 · Morphological expansion of human vs. monkey/ape iPSC-derived astrocytes (iAstrocytes) •

Feb 22, 2024 · In this study, we generated five new chimpanzee iPSC lines from peripheral blood cells and skin fibroblasts with SeV vectors carrying four reprogramming factors (human OCT3/4, SOX2, KLF4, and L-MYC) and characterized their pluripotency and differentiation potential.

Jan 16, 2024 · IPSC technology offers novel approaches and techniques for obtaining pluripotent stem cells (PSCs) from rare and endangered animal species. Successful generation of iPSCs circumvents several bottlenecks that impede the development of PSCs, including the challenges associated with establishing embryonic stem cells, limited embryo sources and ...

Using ape iPSC lines to characterize tolerated variation in gene regulation and developmental processes within primates species may further support interpretation of candidate human disease mechanisms, including those being studied using stem cells and organoid models.

We show that genes and pathways implicated in long-range intercellular signaling are activated in the human iAstrocytes and partake in controlling iAstrocyte complexity. Genes downregulated in human iAstrocytes frequently relate to neurological disorders and were decreased in …

May 1, 2014 · Our results show that primate iPSCs can provide a rich source to identify conserved and species-specific gene expression patterns for cellular phenotypes in humans' closest relatives. iPS cells were generated, cultivated and differentiated using standard procedures described in detail in the Supplemental experimental procedures.