Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …

Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer. Keywords: AOM; Colitis-associated cancer; Colon cancer; DSS; Inflammatory carcinogenesis.

Dec 14, 2020 · In the AOM–DSS model, symptoms are caused by flares of colitis during the cycles of DSS treatment. Intestinal inflammation accompanies changes in body weight and stool consistency.

Mar 24, 2011 · Among the chemically induced CRC models, the combination of a single hit of azoxymethane (AOM) with 1 week exposure to the inflammatory agent dextran sodium sulphate (DSS) in rodents has proven to dramatically shorten the latency time for induction of CRC and to rapidly recapitulate the aberrant crypt foci-adenoma-carcinoma sequence that occurs ...

The combination of DSS with AOM as a model for colitis associated cancer has gained popularity for its reproducibility, potency, low price, and ease of use. Though they have a shared mechanism, AOM has been found to be more potent and stable in solution than DMH.

Furthermore, it was demonstrated that an AOM/DSS model with a low dose of DSS (1%) can be used to reliably induce colorectal carcinogenesis measured as preneoplastic lesions in both B6 and A/J mouse strains while limiting severe symptoms.

Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …

Oct 26, 2023 · This has been a challenge to model in mice with commonly used azoxymethane/dextran sodium sulfate (AOM/DSS), which couples the mutagenic substance AOM with the inflammation-inducing...

May 4, 2020 · In order to explore dynamic regulation of tumor infiltrating ILCs during CRC progression, we used azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colitis-associated CRC, which mimics...

Mar 17, 2023 · Inflammatory bowel diseases (IBDs) contribute to the tumorigenesis of colorectal cancer (CRC). Here, we describe a step-by-step protocol for the construction of colitis-associated CRC murine model by sequential utilization of azoxymethane and dextran sulfate sodium.