ltra ImmunoFix Procedure INTENDED USE The SPIFE Touch Ultra ImmunoFix method is intended for the qualitative iden-tification of monoclonal gammopathies in serum, cerebrospinal fluid (CSF) or urine using protein electrophoresis and imm.

ImmunoFix Controls are used to confirm antisera reactivity. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel.

Kits include: 10 QuickGel IFE Gels, Acid Violet Stain, Tris-Buffered Saline, Citric Acid Destain, QuickGel Blotters, QuickGel IFE Blotter Combs, 20 IFE Templates, and 1 vial each Fixative, IgG, IgA, IgM, Kappa and Lambda antisera.

screening aid for abnormal proteins, but must be used in conjunction with other clinical findings. monospecific SPIFE IFE Antisera (G,A,M,K,L) to identify the abnormal protein. first stage and immunoprecipitation in the second. There are numerous applications for IFE in research, forensic.

Holding the antisera template in the up position, place the template into the appropriate slots in the chamber. Gently lower the antisera template onto the surface of the gel.

Aug 2, 2015 · the chamber lid and press the TEST SELECT/CONTINUE<br /> button. The gel will be predried in the electrophoresis chamber.<br /> NOTE: Do not allow antisera to dry in the template. The Antisera<br /> Template should be cleaned with a mild biocidal detergent. The<br /> template may also be scrubbed with a soft brush to remove any<br /> antisera ...

Clean Applicator, Applicator in this manual, call Helena Laboratories for Base, and Rigid Antisera assistance. Template WARNING: The QuickGel Chamber is factory After each test, clean each reusable piece lubricated.

Abstract: Suitable antisera are essential for use in all immunochemical procedures. Three important properties of an antiserum are avidity, specificity, and titer. The avidity of an antiserum is a measure of the strength of the interactions of its antibodies with an antigen.

Storage and Stability: The antisera should be stored at 2 to 8°C and are stable until the expiration date indicated on the vial. Signs of Deterioration: Extremely cloudy antisera may be indicative of bacterial contamina-tion.

By IFE proteins of sample are first according to their charge separated by electrophoresis on the agarose film, and then they (as antigen) are fixed with monospecific polyclonal antisera.