Antibodies that detect IBA1 can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. These antibodies target IBA1 in Human, Rat and Mouse samples.

Fujifilm Wako’s "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741), which allows even microglia processes to be stained by immunohistochemical staining, is used by researchers all over the world as a microglia marker antibody standard.

Fujifilm Wako's "Anti Iba1, Rabbit (for Immunocytochemistry)" (Product No. 019-19741) is an excellent microglial marker antibody since it can stain even microglial processes. Here, we describes the protocol and points to note when performing microglial immunohistochemistry.

Invitrogen Anti-IBA1 Polyclonal, Catalog # PA5-27436. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (0.14 mg/mL).

IBA1, also known as AIF-1, was first observed in rodent microglial cells and has been shown to stain cells in both normal and pathological lesions in rodents. In addition to the central nervous system, IBA1 has also been shown to stain in macrophage subpopulations.

Rabbit Anti Iba1 antibody is raised against synthetic peptide corresponding to C-terminus of Iba1. Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

Ionized calcium-binding adapter molecule 1 (IBA1), also called the allograft inflammatory factor 1 (AIF1) is mapped to human chromosome 6p21.33. It encodes a 17 kDa protein and comprises calcium binding EF hand motif.