The Dead Cell Apoptosis Kit with Annexin V FITC and PI detects PS externalization in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using PI. Necrotic cells that stain with PI show red fluorescence.

Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability 1,2. The Annexin V/ PI protocol is a commonly used approach for studying apoptotic cells 3.

Annexin V staining is a common method used for identifying and analyzing apoptotic cells, providing valuable insights into the underlaying mechanisms of programmed cell death.

This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis.

FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids.

This Annexin V and PI Staining Apoptosis Assay Protocol for Flow Cytometry can be used to detect phosphatidylserine externalization in the plasma membrane, a key marker of apoptosis.

Because the anticoagulant protein Annexin V binds with high affinity to phosphatidylserine, fluorochrome-conjugated Annexin V can been used to detect apoptotic cells by flow cytometry. Annexin V is commercially available, conjugated to most common fluorochromes.

The Annexin PI assay combines two fluorescent markers, annexin V and propidium iodide (PI), to distinguish between live, dead, and dying cells. Annexin V binds to phosphatidylserine, a marker of early apoptosis, exposing the inner membrane layer.

Jul 15, 2024 · Flow cytometry techniques utilizing dual staining with annexin V and propidium iodide (PI) provide a robust method for quantitatively analyzing apoptosis induction. Annexin V binds phosphatidylserine exposed on the outer leaflet of the plasma membrane during early apoptosis, while PI permeates late apoptotic/necrotic cells.

The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised.