1 Introduction 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA pure system. It also includes relevant guidance for practical handling and maintenance of instrument components. In this chapter This chapter contains the following sections: Section See page...

ÄKTA pure™ is a flexible and intuitive chromatography system (Fig 1) for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product.

1.2 ÄKTApureoverview Introduction ÄKTApureisintendedforpurificationofbio-molecules,inparticularproteins,forresearchpurposes ...

Flexible and intuitive chromatography system for purification in research applications. ӒKTA pure™ chromatography system allows for quick and easy customization due to its modular design. Choose ÄKTA pure™ 25 with a flow rate of up to 25 mL/min or ÄKTA pure™ 150 with a flow rate of up to 150 mL/min.

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User manual for the ÄKTA pure chromatography system. Covers instrument overview, modules, installation, and configuration.

Using bench-scale and pilotscale AKTA chromatography systems, we investigated the impact of individual units within the flow path on ion-exchange chromatography operation.

The diagram represents the automated multistep in-line dilution fluidic scheme of the AKTA pure system. The modules aré labeled with the abbreviations according to the instrument manual.

This site preparation guide covers the ÄKTA avantTM, ÄKTA goTM, ÄKTA pccTM and ÄKTA pureTM systems. The conditions given in this document apply to all systems, unless otherwise specified. In this document, ÄKTA avant, ÄKTA go, ÄKTA pcc, or ÄKTA pure instrument in combi-nation with UNICORNTM software is referred to as the system.

Keep the system and Fraction collector clean, both on the outside and the inside. Use inlet filters and filtered buffers, preferably degassed buffers. For optimal results, use buffers, system and columns at ambient temperature during a purification run.