Dec 15, 2022 · Here we demonstrate that p21 negatively regulates basal autophagy at physiological concentration. Akt activation, upon p21 attenuation, driven ROS accumulation appears to be the major...

Here, we demonstrate that Akt phosphorylates the cell cycle inhibitory protein p21 Cip1 at Thr 145 in vitro and in intact cells as shown by in vitro kinase assays, site-directed mutagenesis, and phospho-peptide analysis.

Here we demonstrate that p21 is directly phosphorylated by AKT/PKB, a survival kinase that is hyperactivated in many late stage tumors. Two sites (Thr 145 and Ser 146) in the carboxyl terminus of p21 are phosphorylated by AKT/PKB in vitro and in vivo.

Mar 29, 2002 · Two sites (Thr(145) and Ser(146)) in the carboxyl terminus of p21 are phosphorylated by AKT/PKB in vitro and in vivo. Phosphorylation of Thr(145) inhibits PCNA binding, whereas phosphorylation of Ser(146) significantly increases p21 protein stability.

Feb 5, 2001 · Here we show that HER-2/neu -mediated cell growth requires the activation of Akt, which associates with p21 Cip1/WAF1 and phosphorylates it at threonine 145, resulting in cytoplasmic...

In this study, we have shown that both Akt and p21 are required to induce cellular senescence in response to p53 expression. In a p53-induced senescence model, we found that Akt activation was essential for inducing a cellular senescence phenotype.

May 22, 2017 · Here, we demonstrated that ER stress activator 3-AWA diminishes the p21 levels in cancer cells by averting the senescent phenotype to commence G2/M arrest. In essence, the deceleration in p21...

Jun 1, 2002 · We cotransfected constitutive activated Akt (CA-Akt) or dominant-negative Akt (DN-Akt) with wild-type or mutant p21 Cip1/WAF1 (T145A and T145D) into p21-/-MEF cells and examined the cellular localization of p21 Cip1/WAF1 by immunofluoresence analysis.

Here, we demonstrate that Akt phosphorylates the cell cycle inhibitory protein p21 (Cip1) at Thr 145 in vitro and in intact cells as shown by in vitro kinase assays, site-directed mutagenesis, and phospho-peptide analysis.

The Akt signaling pathway or PI3K-Akt signaling pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are PI3K (phosphatidylinositol 3-kinase) and Akt (protein kinase B).