These mice can be used in optogenetic studies for rapid in vivo activation of excitable cells by illumination with blue light (450-490 nm). A C57BL/6J congenic version of this strain is available as Stock No. 024109 .

Mar 25, 2012 · These findings demonstrate that reticular neurons can be recruited effectively by local light stimulation in Pvalb-IRES-Cre;Ai32 mice to induce physiologically relevant cortical patterns.

Here we report a low level of leaky ‘off-target’ (Cre-independent) ChR2-EYFP expression in Ai32/Ai32 homozygous mice throughout the nervous system. This leaky off-target expression was characterized in multiple prevalent nervous system regions using anti-EYFP immunostaining.

A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing.

Nov 8, 2023 · Ai32 transgenic mice express channelrhodopsin-2/EYFP (ChR2-EYFP) following exposure to Cre recombinase. Crossing TRAP mice with Ai32 mice makes it possible to produce a stable transgenic line containing both Fos-iCre and ChR2-EYFP.

4 days ago · For optogenetics experiments, we crossed male PV-Cre knockin (The Jackson Laboratory, 017320) mice with female homozygous Ai32 mice (The Jackson Laboratory, 024109) to generate PVxAi32 mice (n ...

May 31, 2016 · Together our characterization of opsin expression, cholinergic cell health and mouse behavior suggest that ChAT-Cre/Ai32(ChR2-YFP) and ChAT-Cre/Ai35(Arch-GFP) mice are promising tools for studying the cholinergic system in mice.

These mice can be used in optogenetic studies for rapid <i>in vivo</i> activation of excitable cells by illumination with blue light (450-490 nm).

Previously, we employed the HDC-Cre;Ai32 mice to examine the consequences of early monocular deprivation on the balance of excitation and inhibition evoked by TC inputs onto pyramidal neurons in L4 of the primary visual cortex (Frantz et al., 2020).

We investigated the photoexcitability of cortical pyramidal neurons from Emx1-Cre;Ai27, Emx1-Cre;Ai32 mice (abbreviated as E-Ai27 and E-Ai32), and Ai32 alone mice not crossed to any Cre, by whole-cell recordings in the barrel cortex of acute brain slices with a previously established photostimulation paradigm 26, 27 , and compared them to ...