These mice can be used in optogenetic studies for rapid <em>in vivo</em> activation of excitable cells by illumination with blue light (450-490 nm).

A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing.

Mar 25, 2012 · We investigated the photoexcitability of cortical pyramidal neurons from Emx1-Cre;Ai27 and Emx1-Cre;Ai32 mice (abbreviated as E-Ai27 and E-Ai32, respectively), and Ai32 alone mice not...

Mar 25, 2012 · Here we present a toolbox of four knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0 and archaerhodopsin Arch-ER2.

Briefly, TH-Cre transgenic mice were crossed with Ai27 mice or were injected with AAV to express ChR2 in their dopaminergic neurons. Photostimulation (5-ms pulses at 20/50 Hz) was applied through an optical fiber connected to a blue laser (λ = 473 nm).

Transgenic mice engineered to express optogenetic tools in a cell type-specific manner offer a powerful approach for examining the role of particular cells in discrete circuits in a defined and reproducible way.

Dec 20, 2011 · expression strategy, we created four new Cre-dependent mouse lines that express optogenetic tools: the H134R mutant of ChR2 (ref. 25) fused to either tdTomato (Ai27; ChR2(H134R)-tdTomato) or...

Oct 15, 2013 · The functionality of ChR2 was demonstrated by photostimulation of cortical pyramidal neurons in brain slices from Emx1-Cre;Ai27 (E-Ai27) and Emx1-Cre;Ai32 (E-Ai32) mice. Detailed comparative analysis revealed stronger light-evoked responses with the E-Ai32 line compared to the E-Ai27 line.

Jul 25, 2023 · The cortical neurons mainly express vesicular glutamate transporter 1 protein (VGlut1). 27 To selectively stimulate these glutamatergic inputs, we crossed VGlut1-Cre mice with ChAT-eGFP and ChR2-tdTomato transgenic mouse line (Ai27) that expresses channelrhodopsin in a Cre-dependent manner. 28 The resulting VGlut1-Cre; ChAT-eGFP; Ai27 mice ...

Driver lines expressing trimethoprim (TMP)-inducible Cre or FlpO. Recombination patterns examined in adult mice (P56 or older) with drivers crossed to either a Cre reporter Ai14 or a Flp reporter Ai65F. Related to. Figure 1. S4. Protein sequence of soma-enriched voltage sensor ASAP2s-Kv. Related to Figure 4.