Exposure to Cre recombinase removes both STOP cassettes, resulting in expression of tTA2 and GCaMP6s (an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). GCaMP6s expression may then be diminished by doxycycline.

Plasmid Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector from Dr. Hongkui Zeng's lab contains the insert GCaMP6s, tTA2 and is published in Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035. This plasmid is available through Addgene.

Mar 23, 2020 · To allow targeting of individual neurons for structural and functional studies, we created light-inducible site-specific DNA recombinases based on Cre, Dre and Flp (RecVs). RecVs can induce...

Jan 6, 2025 · We use the Rbp4-Cre_KL100 line and see delayed growth (postnatally) in Ai148 x Cre mice, but Ai162 x Cre mice seem to be fine. Mice have about 50% size and body weight compared to Bl6 animals at the time of weaning and gain very slowly thereafter.

Nov 12, 2024 · We demonstrate that polytetrafluoroethylene (PTFE)-based inhibition of the postlaminectomy fibrosis preserves long-term spinal cord window clarity, making it possible to record, for months, from...

Jul 12, 2018 · To determine if tTA2 would be better tolerated if expressed later in development, we generated double-transgenic mice containing Ai148, Ai162 or Ai163 and one of pan-cortical Cre lines with later developmental onset, such as Slc17a7-IRES2-Cre, …

Ai162(TIT2L-GC6s-ICL-tTA2)-D (also called Ai162D) mice are a Cre-dependent, Tet-controllable, fluorescent calcium-indicator tool strain - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that …

Strong visual responses were also observed in GCaMP6s-expressing Ai163 and Ai162 lines: in Pvalb- IRES-Cre;Ai163 mice (3 mice, 18 FOVs), for example, responses in PV neurons were on average ~5...

Hi, I'm looking to use the new Ai162 transgenic reporter to cross into my PV-Cre driver line for wide field calcium imaging (Ai93 line does not express well enough in interneurons).

Driver lines expressing trimethoprim (TMP)-inducible Cre or FlpO. Recombination patterns examined in adult mice (P56 or older) with drivers crossed to either a Cre reporter Ai14 or a Flp reporter Ai65F. Related to. Figure 1. S4. Protein sequence of soma-enriched voltage sensor ASAP2s-Kv. Related to Figure 4.