Ai14 mice express robust tdTomato fluorescence following Cre-mediated recombination.

Using previously reported and novel lipid nanoparticle vectors, we deliver mRNA encoding for Cre protein to Ai14 mice, which express tdTomato upon Cre recombination (Figure 1). When the same...

Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt (ROSA)26Sor locus.

5 days ago · The mice used in the study included vGluT2-Cre;Ai14 and vGat-Cre;Ai14 mice, both of which expressed oChIEF-tdtomato, as well as two control groups: Cre mice expressing tdtomato and Cre mice ...

Mar 14, 2024 · To achieve astrocyte-specific recombination in floxed Ai14 reporter mice, we intravenously injected blood-brain barrier–penetrating AAV-PHP.eB vectors expressing Cre recombinase (Cre) using the astrocyte-specific mouse …

Jun 13, 2024 · To investigate the potential of lung SORT LNPs to deliver mRNA-encoded gene editors to lung stem cells (18, 21 – 23), we used Ai14 LoxP-stop-LoxP tdTomato (tdTom) reporter mice (24), in which removal of the stop cassette orchestrated by Cre recombinase or CRISPR-Cas9 activates tdTom expression, allowing identification of gene-edited cells (Fig. ...

Using Ai14 and Ai3, two commonly used fluorescent reporter genes, we show that those basal Cre activity levels are sufficient to label a significant amount of cells in a variety of tissues during embryogenesis, postnatal development and adulthood.

Using a well-established mouse model of cancer cachexia, ... To trace the ArP→PBN projection, we injected AAV1-hSyn-Cre (1.8 × 10 13 GC/ml, 90 nl, Addgene) into ArP of Ai14 mice. The PBN cell bodies that receive projections from ArP are visible due to transsynaptic tracing by AAV1. To trace ArP→PBN projections, CAV2-Cre was injected in PBN ...

Feb 20, 2020 · ECSF mice were crossed to Ai14 mice, which have a LoxP-flanked stop cassette excisable by Cre (upper). The ECSF; Ai14 progeny were injected with tamoxifen or vehicle, and tail preparations were PCRed using primers flanking the Ai14 LoxP sites.

May 1, 2020 · To apply PA-Cre 3.0 to mammalian primary cells, we transduced this construct into mouse primary neural progenitor cells isolated from Ai14: Floxed-tdTomato heterozygous reporter mouse brains.