SUMO protease recognizes the sequence x-Gly-Gly-x, and therefore doesn’t cleave the target protein. This aspect of SUMO proteases make SUMO an attractive affinity tag because no …

The N-terminus of SUMO is very flexible and can accommodate a variety of affinity tags (e.g., GST). The SUMO system can therefore be tailored to the researcher’s desired affinity tag.

Jan 10, 2012 · Affinity tags are highly efficient tools for protein detection, characterization, and purification. An epitope is a short sequence of amino acids that typically serves as the …

Here, we describe a tandem-tag purification method that offers a remedy to this problem. It contains only common affinity-chromatography steps (HisTrap and Heparin) to ensure low …

The SUMO tag will be firstly incorporated with an affinity tag then expressed together with the target protein by the host, followed by enzyme activity to detach the SUMO tag. During …

SUMO attachment can be removed by SUMO-specific isopeptidases just as UCHs and UBPs remove ubiquitin from substrate proteins. Efficient removal of the SUMO tag by SUMO …

SUMO can not only be used as a fusion tag for recombinant protein expression but also functions as a molecular chaperone to promote the correct folding of proteins. SUMO has a tolerance to …

Oct 12, 2016 · Many tag-fusion expression vectors are now available for the purposes of enhancing solubility, enabling one-step affinity-based purification and facilitating the detection …

Dec 15, 2016 · Genetically modified solubility-enhancing and affinity peptide fusion technology has immensely aided the enhanced expression and efficient purification of functional …

Dec 11, 2014 · Arguably the simplest affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, …