Affinity labels are a class of enzyme inhibitors that covalently bind to their target causing its inactivation. The hallmark of an affinity label is the use of a targeting moiety to specifically and reversibly deliver a weakly reactive group to the enzyme that …

Affinity labeling is a technique for labeling the binding (or active) site of proteins by virtue of a ligand analog to which a chemically reactive or photoreactive group has been attached. The latter can form a covalent bond with a suitably oriented amino acid in the binding (active) site.

Photoaffinity labeling is a recently introduced method for covalently binding chemical tags to the active sites of protein molecules, which is potentially capable of very great specificities of labeling.

Jan 1, 1977 · Affinity labeling by definition applies to all molecules possessing a site that binds another molecule with a certain degree of specificity and affinity. In biology the proteins are the obvious ligand-binding molecules, and in practice the affinity labeling techniques have primarily involved proteins.

Affinity labeling is a technique for labeling the binding (or active) site of proteins by virtue of a ligand analog to which a chemically reactive or photoreactive group has been attached.

The strategy of affinity labeling restricts the extent of chemical modification to the region of the catalytic site by exploiting the specificity of the enzyme for its natural substrate: a reagent is designed that is related structurally to the normal substrate or regulatory compound of the enzyme but that also features a functional group ...

Nov 30, 2023 · In this study, a novel approach to traceless affinity-directed intact protein labeling is established, which leverages small binding proteins and genetic code expansion technology.

Photoaffinity labeling is a chemoproteomics technique used to attach "labels" to the active site of a large molecule, especially a protein.

Affinity labeling can indicate, for an enzyme in solution, that there is proximity between the modified amino acid and the natural ligand that is mimicked by the affinity reagent; it does not prove that the target amino acid makes a direct contribution to catalysis, regulation, or binding.

Affinity labeling is a technique for the specific modification of an amino acid residue in a protein which involves both the binding of the reagent (affinity label) on the basis of biological specificity and subsequent modification of an amino acid residue through the formation of a covalent bond.