Adenovirus vectors are the most commonly employed vector for cancer gene therapy. They are also used for gene therapy and as vaccines to express foreign antigens. Adenovirus vectors can be replication-defective; certain essential viral genes are ...

There are 57 accepted human adenovirus types, and most adenoviral vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus Receptor (CAR) to enter cells. Recombinant adenovirus has two genes deleted: E1 and E3.

The adenovirus vector platform remains one of the most efficient toolboxes for generation of transfer vehicles used in gene therapy and virotherapy to treat tumors, as well as vaccines to protect from infectious diseases.

An Adenovirus Vector is a type of viral vector commonly used in gene therapy and vaccine development due to its high transgene expression levels in various host cells. These vectors are typically nonintegrating, double-stranded DNA viruses that have been genetically modified for reduced toxicity and improved gene delivery.

Adenoviral vector is considered to be a promising delivery vehicle for in vivo gene editing. Palmer et al. developed a single helper-dependent Ad (HDAd), which is used to deliver all the components of CRISPR-Cas9 for efficient gene editing in transduced cells.

Since the isolation of adenovirus (AdV) in 1953, AdVs have been used as vectors for various therapeutic purposes, such as gene therapy in cancers and other malignancies, vaccine development and delivery of CRISPR-Cas9 machinery.

Adenovirus vectors offer many advantages for gene delivery: they are easy to propagate to high titers, they can infect most cell types regardless of their growth state, and in their most recent embodiments they can accommodate large DNA inserts.

Feb 15, 2021 · In this review, I discuss the innate and adaptive aspects of the immunological characteristics of adenovirus vectors and further examine the current status of advanced adenovirus-based vaccine development.

In this review, we summarize the biology of adenovirus vectors, their manufacturing methods, and the origins of RCA formed during HEK293-based vector production. Lastly, we share our experience using minimally RCA-positive serotype 5 adenoviral vectors based on observations from our clinical cardiovascular gene therapy studies.

Currently, the adenovirus vector (AV) is the most commonly used vehicle for locally administered BMP gene therapy because of its high efficiency in infecting both dividing and non-dividing cells (Chen et al., 2003).