Adeno-associated viruses (AAV) are small viruses that infect humans and some other primate species. They belong to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae.

As part of its lysogenic cycle, wild-type AAV integrates into the host genome at a specific site, AAVS1 on human chromosome 19. This site is favored due to the presence of a Rep binding element; however, random integrations may occur at a much lower frequency. As a replication-incompetent virus, AAV cannot enter the lytic cycle without help.

Viruses that spread transsynaptically provide a powerful means for studying interconnected circuits in the brain. Here we describe the use of adeno-associated virus, serotype 1 (AAV1) as a tool for achieving robust, anterograde transsynaptic spread in a variety of unidirectional pathways.

Jan 16, 2025 · AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce skeletal muscle, cardiac, and retinal cells (Issa et al., 2023).

Moreover, recombinant AAV1 (rAAV1) was not found to contain any detectable post-translational modifications (PTMs), according to a systematic analysis conducted on ten AAV serotypes by Mary B. et al. , and it was the first viral vector to be approved for use in gene therapy .

The bioluminescence imaging revealed three expression levels (i) low-expression group, AAV2, 3, 4, and 5; (ii) moderate-expression group, AAV1, 6, and 8; and (iii) high-expression group, AAV7 and 9. In addition, imaging revealed two classes of kinetics (i) rapid-onset, for AAV1, 6, 7, 8, and 9; and (ii) slow-onset, for AAV2, 3, 4, and 5.

May 13, 2013 · AAV5 is the most divergent (55% similarity vs. other serotypes) and like AAV1 vector is known to transduce liver efficiently. AAV1 and AAV5 vectors are also immunologically distinct by virtue...

In the present study, we sought to compare the biologic properties of rAAV2/1, rAAV2/2, and rAAV2/5 transduction in polarized human airway epithelia using viruses purified by a newly developed common column chromatography method.

May 12, 2016 · For AAV serotype 1 (AAV1), the first viral vector approved as a gene therapy treatment, and its closely related AAV6, sialic acid (SIA) serves as their primary cellular surface receptor. Toward characterizing the SIA binding site(s), the structure of the AAV1-SIA complex was determined by X-ray crystallography to 3.0 Å.

Sep 24, 2013 · AAV1/SERCA2a indicates recombinant adeno-associated viral vector (AAV) containing human sarcoplasmic reticulum Ca 2+ ATPase 2a (SERCA2a) gene; DRP, DNase-resistant particles; HF, heart failure; LTFU, long-term …