Plasmid AAV-hSyn-EGFP from Dr. Xue Han's lab contains the insert Enhanced Green Fluorescent Protein and is published in Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. This plasmid is available through Addgene.

Jun 23, 2020 · The mPGK promoter did drive expression in cortical neurons and oligodendrocytes, while transduction with AAV harbouring the hSYN promoter resulted in neuron-specific expression, including...

Viruses that spread transsynaptically provide a powerful means for studying interconnected circuits in the brain. Here we describe the use of adeno-associated virus, serotype 1 (AAV1) as a tool for achieving robust, anterograde transsynaptic spread in a variety of unidirectional pathways.

May 27, 2024 · We tested this strategy by transfecting mouse Neuro2a (N2a) cells with a ConVERGD construct expressing enhanced green fluorescent protein (eGFP) (pAAV-hSyn-ConVERGD-eGFP-W3SL) alone or...

Feb 7, 2023 · AAV-hSyn-INTRSECT-Con/Fon-EYFP was injected into the LC of Pdyn Cre, Dbh Flp, and Pdyn Cre;Dbh Flp mice at the same titer used for ConVERGD injections (~7.87E11 gc/mL). Under these conditions, we observed a considerable number of INTRSECT labeled neurons in Pdyn Cre;Dbh Flp samples.

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control.

hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

We have characterized the transduction of mouse somatosensory cortical neuron types by recombinant AAV pseudotyped with serotype 1 capsid (rAAV2/1) and by recombinant lentivirus pseudotyped with the VSV glycoprotein. Both vectors utilized human synapsin (hSyn) promoter driving DsRed-Express.

Jan 4, 2017 · AAV1-Cre from transduced presynaptic neurons effectively and specifically drives Cre-dependent transgene expression in selected postsynaptic neuronal targets, thus allowing axonal tracing and functional manipulations of the latter input-defined neuronal population.

Jun 20, 2003 · Two short promoters, the human synapsin 1 gene promoter (hSYN) and the murine cytomegalovirus immediate early promoter (mCMV), were evaluated in bicistronic AAV-2 vectors for their expression profiles in cultured primary brain cells and in the rat brain.