Feb 19, 2024 · Analytical ultracentrifugation (AUC) Quantification of percent full, intermediate, and empty AAV capsids was performed using an Optima AUC (Beckman Coulter).

Analysis of AUC data can be performed with or without the application of modeling-based approaches to extract particle properties. A review by Edwards et al. 202013 listed the …

Here, we demonstrate the novel application of analytical ultracentrifugation (AUC) to characterize the homogeneity of preparations of rAAV vectors. We show that a single sedimentation …

Here we demonstrate that mass photometry can be used as an orthogonal method to AUC to accurately quantify the proportions of empty, partially filled, full and overfull particles in AAV …

Validating an AUC method for the characterization of AAV samples, for QC testing and potential lot release is a large undertaking, whereby precision, linearity, and accuracy are but a few of …

The goal of this work is to evaluate analytical ultracentrifugation (AUC) as a means of characterizing one particular type of gene delivery vector: recombinant adeno-associated virus …

Here, we report the applications and limitations of equilibrium density gradient analytical ultracentrifugation (DGE-AUC) using a modern AUC instrument that employs DGE-UC …

Mar 11, 2024 · The recent increase in the development of adenovirus-associated virus (AAV) based gene therapies has created a large demand for the analysis of AAV-based drugs for the …

May 28, 2023 · Due to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors, boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has …

A single AUC experiment provides insights into the composition of the encapsulated DNA, the success of the purification, the presence of aggregates and the ratio between empty, partially …