Aug 1, 2023 · Here, we show that ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane (PM). Through shRNA screening, we identified ARF6 as the key small GTPase in targeting CD36, a palmitoylated protein, from the Golgi to the PM.

Oct 12, 2022 · ARF6 recognizes palmitoylated EGFR with its N-myristoylation, recruits the exocyst complex with Lys3 to promote EGFR budding from Golgi, and gets converted to GTP-bound form by EFA6B to...

May 1, 2006 · ARF6 is the least-conserved member of the ARF family of proteins and shares 66% amino-acid identity with ARF1, but unlike ARF1, ARF6 has no effect on Golgi membrane dynamics.

The activation of the small GTPase ARF6 has been implicated in promoting several pathological processes related to vascular instability and tumor formation, growth, and metastasis. ARF6 also plays a vital role during embryonic development. Recent ...

May 18, 2011 · Now, new roles for ARFs have been discovered at the Golgi complex, for example in driving lipid transport. ARL proteins are also being increasingly linked to coordination of trafficking with...

Jan 1, 2022 · Class I and II Arfs mainly localize to the Golgi, but also to endosomes and/or the ER–Golgi intermediate compartment (ERGIC), whereas Arf6 is found in the cell periphery. Arfs are ubiquitously expressed, but vary in their abundance (Cavenagh et …

In the present study we have shown that ARF proteins belonging to the three classes, ARF1, ARF5 and ARF6, can interact with all GGA proteins in a yeast two-hybrid assay, in vitro and in vivo.

Arf6 influences membrane trafficking and the actin cytoskeleton at the plasma membrane (PM). The goal of this review is to summarize these recent findings and provide a cellular context for understanding Arf6 function.

Jul 18, 2023 · Through a shRNA screening, we identify ARF6 as the key small GTPase in targeting CD36, a palmitoylated protein, from Golgi to PM. The N-terminal myristoylation of ARF6 is required for its...

In cells, myristoylation is required for correct localization of Arf proteins, including Golgi localization of Arf1 and PM localization of Arf6 (Donaldson and Jackson 2011). In vitro, the myristoyl group of the GDP-bound form of Arf1 is available for membrane insertion, permitting a weak association of the inactive form of Arf1 with membranes ...