Feb 22, 2023 · Our goal was to provide a analysis of Arf6’s function in angiogenic endothelium, focusing on its role in lumenogenesis as well as its relation to actin and CME. We found that Arf6 localized to both filamentous actin and sites of CME in 2-dimensional culture.

May 1, 2006 · ARF6 localizes to the plasma membrane and endosomal compartments, in which it regulates clathrin-dependent endocytic membrane trafficking, as well as the trafficking and sorting of molecules that...

Apr 2, 2020 · By reconstituting actin dynamics on supported lipid bilayers, we found that Arf6 in co-ordination with Rac1 (Ras-related C3 botulinum toxin substrate 1) can directly trigger actin polymerization by recruiting wave regulatory complex components.

The small GTPase, ADP-ribosylation factor-6 (ARF6), has been implicated in regulating membrane traffic and remodeling cortical F-actin. Using real-time video analysis of actin assembly in living cells, we investigated the function and mechanism of ARF6 in control of actin assembly.

Mar 23, 2015 · Here we show that overexpression of an ARF6 fast-cycling mutant in MDA-MB-231 breast cancer-derived cells to mimick ARF6 hyperactivation observed in invasive breast tumors induced a striking rearrangement of the actin cytoskeleton at the ventral cell surface.

Oct 1, 2013 · We demonstrate that Arf6 triggers actin assembly at the membrane indirectly by recruiting the Arf g uanine nucleotide exchange factor (GEF) ARNO that activates Arf1 to enable WRC-dependent actin assembly.

Sep 26, 2023 · ADP ribosylation factor 6 (Arf6) is a small GTPase that promotes the assembly of actin necessary for clathrin-mediated and clathrin-independent endocytosis. In its absence, growth factor signaling is greatly diminished, which has been shown to ameliorate pathological signaling input in diseased vasculature.

Oct 1, 2023 · ADP-ribosylation factor 6 (Arf6), a small G-protein of the Ras superfamily, plays pivotal roles in multiple cellular events, including exocytosis, endocytosis, actin remodeling, plasma membrane reorganization and vesicular transport.

Arf6 influences membrane trafficking and the actin cytoskeleton at the plasma membrane (PM). The goal of this review is to summarize these recent findings and provide a cellular context for understanding Arf6 function.

Here, we investigated the role of the Arf6 GAPs in Arf6-mediated cortical actin cytoskeleton remodeling and studied the importance of the completion of the full GDP/GTP cycle of Arf6 for the post-endocytic transport of major histocompatibility complex class I (MHCI).