Jan 15, 2021 · In this study, because Arf1 and Arf6 are the best-characterized members among Arf family, we established T lineage-specific Arf1-deficient, Arf6-deficient, and Arf1/6 double-deficient mice to understand physiological roles of the Arf pathway in the immune system.

May 1, 2006 · ARF6 localizes to the plasma membrane and endosomal compartments, in which it regulates clathrin-dependent endocytic membrane trafficking, as well as the trafficking and sorting of...

Arf1 triggers budding of coated carrier vesicles from the Golgi complex and endosomal compartments. Arf6 is divergent from Arf1–Arf5 and localizes to the plasma membrane and endosomal compartments, where it regulates endosomal recycling and remodelling of actin cytoskeleton and membranes (5–7).

Nov 1, 2023 · Here, we identify the GTPase ADP-ribosylation factor 1 (ARF1) as a crucial negative regulator of cGAS-STING signalling. Heterozygous ARF1 missense mutations cause a previously unrecognized...

Arf6 and Arf1 activates PLD1 at the cell plasma membrane or cis Golgi, respectively. Arf6 activates PIP5Ks to generate PI(4,5)P 2 in the plasma membrane. Arl5a and Arl5b recruit PI4KB to the TGN and promote the local PI4P generation.

ARF1-5 regulate vesicle budding at the Golgi apparatus by recruiting coat complexes (Li et al., 2004; Kahn et al., 2006). ARF6 localizes to the plasma membrane, as well as endosomes and is involved in actin cytoskeleton dynamics and endocytic recycling ( Donaldson, 2003 ).

Mar 30, 2025 · ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D.

We have also modeled ARF1 onto the ARF6 lattice, and then pursued vesicle reconstitution by the Coat Protein I (COPI) complex to further confirm that the ARF lattice acts in vesicle fission. By elucidating how an ARF protein tubulates membrane at the structural level, we have advanced the molecular understanding of how this class of transport ...

Arf6 influences membrane trafficking and the actin cytoskeleton at the plasma membrane (PM). The goal of this review is to summarize these recent findings and provide a cellular context for understanding Arf6 function.

Aug 11, 2014 · By analyzing the regulation of EFA6A activity toward Arf1 and Arf6 using nucleotide exchange reactions reconstituted on artificial membranes, this study reveals unexpected commonalities and divergences among the three ArfGEF subfamilies, yielding a framework for envisioning how they work on peripheral membranes.