Dec 28, 2024 · We evaluated the potential beneficial impact of AMPK-ULK1 activation by Metformin (Met) on mitochondria defects, as well as on early- and late-stage AD-related alterations in vivo and ex vivo.

Dec 28, 2024 · ULK1 cascade on mitochondria structure and functions in APPswe cells. We evaluated the potential beneficial impact of AMPK-ULK1 activation by Metformin (Met) on mitochondria l as on early- and late-stage AD-r Results: At first, we show that AMPK-ULK1 cascade is defective in murine and human AD

Mar 21, 2025 · AMPK is rapidly activated following any stress that triggers a lowering of intracellular ADP/ATP ratio, which initiates ULK1 phosphorylation and the downstream components of autophagy cascade (40, 41).

AMPK/ULK1 Axis in Mitophagy Cascade In response to mitochondrial damage, as well as under energetic stress, the AMPK complex (consisting of a catalytic α, a scaffolding β, and a regulatory γ subunit) acts as a sensor.

Dec 28, 2024 · We designed this study to investigate AMPK-ULK1 activation state in post-mortem human sporadic AD brains, in 3xTgAD mice that recapitulate most of human AD features, and in neuronal cells expressing the amyloid precursor protein with the …

Jan 23, 2011 · Here we demonstrate a molecular mechanism for regulation of the mammalian autophagy-initiating kinase Ulk1, a homologue of yeast ATG1. Under glucose starvation, AMPK promotes autophagy by...

ULK1 catalytic activity is regulated in bi-modally by AMPK: by direct phosphorylation of at least four different sites and via AMPK-dependent suppression of mTORC1 inhibitory phosphorylation of ULK1.

Jan 1, 2025 · Here, we summarize our current understanding of the role of AMPK/ULK1 signaling in regulating different steps of mitophagy, focusing on Parkin activation, autophagosome formation, mitochondrial dynamics, spatiotemporal actions, and the chance of counteracting pathological phenotypes.

We examined the impact of the pharmacological and genetic modulation of AMPK-ULK1 cascade on mitochondria structure and functions in APPswe cells. We evaluated the potential beneficial impact of AMPK-ULK1 activation by Metformin (Met) on mitochondria defects, as well as on early- and late-stage AD-related alterations in vivo and ex vivo.

Feb 25, 2021 · In the present study, we identified that inactivation of AMPK-ULK1 signaling cascade mediated protective autophagy sensitized BC cells to doxorubicin in vitro. Methods: Cell counting kit-8 (CCK-8) assay and colony formation assay were performed to evaluate cell proliferation abilities.