We explored the possibility that membrane CD36 signaling might influence AMPK activation. We show, using several cell types, including myocytes, that CD36 expression suppresses AMPK, keeping it quiescent, while it mediates AMPK activation by FA.

In summary, this study documents the close coordination between CD36 and AMPK in the regulation of cellular FA metabolism. CD36 maintains basal AMPK quiescence, whereas FA-induced CD36 signaling activates AMPK, which disinhibits β-oxidation and recruits more CD36 to the sarcolemma.

CD36 knockdown in HepG2 cells increased lipophagy and β-oxidation, which contributed to improving lipid accumulation. In addition, CD36 expression regulated autophagy through the AMPK pathway, with phosphorylation of ULK1/Beclin1 also involved in the process.

Nov 7, 2023 · For example, we found that CD36 is induced downstream of lipotoxic stress by p38 and AMPK, kinases that both play a role in mitigating energetic stress, and that CD36 deletion further increased phosphorylation of AMPK and p38.

Jan 28, 2025 · Thus, CD36 is sufficient to overcome AMPK deficiency to promote FAO. Finally, ectopic expression of CD36 decreased 4-HNE staining induced by AMPK KO in the primary tumors, demonstrating that CD36 rescues oxidative stress in vivo (Figure 4I).

Our study documents that AS160 mediates insulin or AMPK-stimulated surface translocation of CD36 in cardiomyocytes. Knock-down of AS160 redistributes CD36 to the surface and abrogates its translocation by insulin or the AMPK agonist 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR).

Apr 1, 2019 · CD36 expression maintains AMPK quiescence by allowing Fyn to access and phosphorylate LKB1, promoting its nuclear sequestration away from AMPK. LCFA binding to CD36 activates AMPK within minutes via its ability to dissociate Fyn from the complex as CD36 is internalized into LKB1-rich vesicles.

Apr 19, 2022 · CD36 is a type 2 cell surface scavenger receptor widely expressed in many immune and non-immune cells. It functions as both a signaling receptor responding to DAMPs and PAMPs, as well as a long chain free fatty acid transporter.

The FA translocase cluster of differentiation 36 (CD36) facilitates FA uptake by the myocardium, and its surface recruitment in cardiomyocytes is induced by insulin, AMP-dependent protein kinase (AMPK), or contraction.

Aug 19, 2014 · We explored the possibility that membrane CD36 signaling might influence AMPK activation. We show, using several cell types, including myocytes, that CD36 expression suppresses AMPK, keeping it quiescent, while it mediates AMPK activation by FA.