Mar 26, 2003 · We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB.

Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify …

Sep 15, 2020 · The AFLP method not only needs background knowledge on the targeted genome but also has high reproducibility and sensitivity for finding polymorphism at various levels of the DNA sequence while provides valuable information in various loci of the targeted genome.

Jul 1, 2003 · We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked...

To accomplish this, the AFlP (amplified fragment length polymorphism) technique was first optimised. The results indicated that the Mlul/Msel restriction enzyme combination produces a higher percentage of polymorph isms when compared to the PstllMsel enzyme combination.

Amplified fragment length polymorphism (AFLP) DNA fingerprinting is a firmly established molecular marker technique, with broad applications in population genetics, shallow phylogenetics, linkage mapping, parentage analyses, and single-locus PCR marker development.

Based on selective PCR amplification of restriction fragments from digested DNA. Highly sensitive method for fingerprinting DNA of any origin and complexity. Can be performed with total genomic DNA or with cDNA ('transcript profiling')

The AFLP® Analysis System I has been designed for use with plants having genomes ranging in size from 5 × 10 8 to 6 ×10 9 bp, including tomato, corn, soybean, cucumber, lettuce, barley, cotton, oilseed rape, potato, sunflower, pepper, and brassica.

Amplified fragment length polymorphism (AFLP) is a PCR-based technique that involves restriction of genomic DNA, followed by ligation of adapters to the fragments generated and selective PCR amplification of a subset of these fragments 1,2.

Abstract: Amplified fragment length polymorphism (AFLP) datasets have been used to construct neighbor-joining trees from which monophyletic origins for crops such as einkorn wheat, barley, and emmer wheat have been inferred.