Adeno-associated viruses (AAV) are small viruses that infect humans and some other primate species. They belong to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae.

May 13, 2013 · AAV5 is the most divergent (55% similarity vs. other serotypes) and like AAV1 vector is known to transduce liver efficiently. AAV1 and AAV5 vectors are also immunologically distinct by virtue of...

We found that a divergent AAV serotype, AAV5, binds and infects from the apical surface of human airway epithelia more efficiently than AAV2 (52). Moreover, AAV5 requires sialic acid for binding and infection (26, 46) rather than the heparan sulfate proteoglycan used by AAV2.

For example, AAV2/5 targets neurons that are not efficiently transduced by AAV2/2, and is distributed more widely in the brain, indicating improved transduction efficiency. Many of these hybrid viruses have been well characterized and may be preferred over standard viruses for in vivo applications.

Jun 14, 2022 · AAV5 is a unique serotype featured by low neutralizing antibody prevalence. Nevertheless, its liver infectivity is relatively weak. Consequently, it is vital to exploit novel AAV5 capsid mutants with robust liver tropism.

The drug is an AAV serotype 5 (AAV5), carrying the gene of deficient factor IX. As for hemophilia type A, Roctavian has also recently been approved by the FDA as an AAV5-based gene therapy vector, carrying the deficient factor VIII gene, driven by a liver-selective promoter [ 1 ].

Mar 12, 2021 · AAV5 has a distinct advantage in evading pre-existing neutralizing antibodies but is hampered by poor transduction efficiency. To improve AAV5-based liver gene therapy, we evolved and screened AAV5 mutants on human liver cell lines. The result is that more efficient human liver tropic AAV5 capsids retained low humoral seroreactivity.

May 1, 2022 · High-resolution cryogenic electron microscopy (cryo-EM) structures detail adeno-associated virus (AAV) receptor protein binding interactions. AAV’s glycan 'primary receptors' are recharacterized as 'attachment factors'. AAV5-like viruses interact with a different domain of the receptor using a binding site that is distinct from that of other AAVs.

Mar 21, 2022 · It highlights the significance of AAV-mediated gene delivery to liver. AAV5 is a unique serotype featured by low neutralizing antibody prevalence. Nevertheless, its liver infectivity is relatively weak. Consequently, it is vital to exploit novel …

The adeno-associated viruses (AAVs) display differential cell binding, transduction, and antigenic characteristics specified by their capsid viral protein (VP) composition. Toward structure-function annotation, the crystal structure of AAV5, one of the most …