AAV transduction of ND and D C2C12 cells. C2C12 cells were seeded onto 96-well plate at density of 5,000 cells/well and, after 24 hours, transduced with AAV6-EGFP or AAV2-Luciferase at MOIs of 1 × 10 4, 5 × 10 4, and 1 × 10 5 vg. Cells were passaged 2 days after plating when they reached confluence.

Encapsidation of rAAV-2 genome in AAV-5 capsid enhances transduction in differentiated, but not undifferentiated, C2C12 cells. C2C12 cells are myoblast cells derived from the C3H strain of mice which can differentiate into contractile myotubes and produce muscle-specific proteins.

Sep 19, 2019 · However, as we show here using primary mouse hepatocytes and skeletal muscle-derived C2C12 cells, AAV2-DJ efficiently infects cells in vitro.

Gene therapy vectors based on the adeno-associated virus (AAV) are extremely efficient for gene transfer into post-mitotic cells of heart, muscle, brain, and retina. The reason for their...

To validate the targeting effect of the muscle-targeting peptide for AAV-mediated gene transfer, differentiated C2C12 myotubes were infected with modified and wild-type vectors harboring a luciferase reporter gene. Gene transfer was enhanced 2- to 3-fold compared to AAV vectors carrying wild-type capsid.

May 28, 2009 · Differentiated C2C12 myotubes were infected with unmodified AAV2, AAV 587 MTP and AAV 588 MTP containing a luciferase report gene in the presence of soluble heparin, which is known to ...

A common lipid-based transfection reagent was used (Lipofectamine 2000) along with three different viral vectors: adeno-associated virus serotype 2 (AAV2), baculovirus (BAC) and lentivirus. Maximal transfection efficiencies of 49% were obtained in C2C12 cells after optimizing cell density and reagent : DNA ratio, although the GFP signal rapidly ...

The C2C12 mouse myoblast cells were cultivated to verify that lncDLEU2 inhibits muscle proliferation and differentiation by acting as a miR-181a sponge regulated SEPP1.

Mar 6, 2013 · We transduced five different mammalian cell lines, including murine 3 T3 cells, murine C2C12 cells, murine MIN6 cells, Chinese hamster ovary cells (CHO), and monkey COS-7 cells with the ten different AAV serotypes and evaluated %GFP + …

Nov 4, 2005 · For example, even though AAV1 and AAV2 do not transduce C2C12 muscle cells efficiently in culture, mosaic AAV1/2 virions, produced by transfection of AAV1 and 2 helper constructs at a ratio of 1:19, exhibited dramatically increased transduction in C2C12 cells.