We first examined the tissue and cell specificity of AAV8-TBG using mice homozygous for the R26-LSL-tdTomato allele on a C57BL/6 background by simultaneous injection with AAV8-TBG-Cre and AAV8-TBG-GFP (herein referred to as AAV-Cre and AAV-GFP, respectively) (Fig. 1A).

Ready-to-use AAV8 particles produced from AAV.TBG.PI.Cre.rBG (#107787). In addition to the viral particles, you will also receive purified AAV.TBG.PI.Cre.rBG plasmid DNA. TBG-driven expression of Cre. These AAV preparations are suitable purity for injection into animals.

Sep 15, 2021 · Adeno-associated virus 8 (AAV8) vectors are valuable instruments for the manipulation of hepatocellular gene expression. However, their off-target effects in mice have not been thoroughly explored. Here, we sought to identify the short-term off-target effects of AAV8 administration in mice.

Jan 12, 2024 · Diagram of rAAv transduction pathway. Adeno-associated virus (AAV) is recognized by glycosylated cell surface receptors of the host. This triggers the internalization of the virus via clathrin-mediated endocytosis. AAV then traffics through the cytosol mediated by the cytoskeletal network.

May 19, 2020 · Ø TBG启动子是一种基于人甲状腺结合球蛋白 (TBG)启动子和微球蛋白增强子的混合型启动子，用于肝脏特异性转外源基因表达。 Ø Cre重组酶是在P1噬菌体中发现的一种重组酶，具有催化活性，而且与限制酶相似，能识别特异的DNA序列，即LoxP位点。 Cre重组酶作用于同一DNA链上两个同方向的LoxP位点时，可以有效删除两个LoxP位点之间的碱基序列，达到重组靶DNA链的目的。 Ø AAV8-TBG-Cre腺相关病毒可高效快速感染小鼠肝脏，目前被认为是最特 …

Mar 12, 2024 · TBG（Thyroxine binding globulin）是肝脏特异表达的一个54 kDa的酸性糖蛋白，主要功能是在循环系统中结合携带甲状腺激素，这个基因的启动子结构早在三十年前就已经研究透彻，在此理论基础上精简后的 TBG启动子 被广泛应用在AAV的载体上。 TBG作为AAV的启动子能在肝细胞中长期的持续过表达。 TBG特异性使目的基因或者干扰序列低表达在其他组织，可以减少对其他组织器官的影响，同时也降低了对其他器官的毒性或者免疫原性，降低实验的干扰。 …

To deliver AAV-Cre, a stock solution of AAV8-TBG-Cre (Addgene 107787-AAV8) was diluted in PBS to a total volume of 20 μL and injected intraperitoneally into postnatal day five.

Dec 11, 2024 · aav-tbg-egfp $ 299.00 – $ 1,799.00 The TBG (thyroxine-binding globulin) promoter is a liver-specific promoter commonly used in gene therapy and gene expression studies to drive the expression of transgenes specifically in liver cells (hepatocytes).

AAV8-TBG-GFP is a pre-packaged rAAV in serotype 8 (capsid from AAV serotype 8 and 2xI TR from AAV serotype 2) which over-ex presses EGFP under the liver specific TBG promoter. TBG promoter is a hybrid promoter based on the human thyroid hormone-binding globulin (TBG) promoter and microglobin/bikunin enhancer; The TBG promoter is about 0.8 kb in ...

You can produce AAV paticle in 293T cell line in high titer using GeneMedi's AAV expression vector (overexpression, shRNA or Crispr-cas9-gRNA) with GeneMedi's serotypes-specific AAV Rep-Cap plasmids and AAV helper plasmid thyroxine binding globulin (TBG) promoter.