Sep 19, 2019 · Among viral delivery systems, adeno-associated viruses (AAVs) are relatively safe and demonstrate high gene transfer efficiency, low immunogenicity, stable long-term expression, and...

Feb 4, 2021 · In the current study, a new series of equations for SEC-MALS are used in tandem with intrinsic properties of the capsids and encapsidated DNA to quantify multiple physical AAV attributes in one...

Here we present optimized size-exclusion chromatography (SEC) methods that can separate soluble AAV self-associated forms and fragments under non-denaturing conditions for several CMV-GFP control AAV serotypes including AAV1, AAV2, AAV5, AAV6, AAV8, and AAV9.

Jul 17, 2019 · We took adeno-associated virus (AAV) as an example and showed benefits of combined use of molecular methods and transmission electron microscopy (TEM) for viral vectors' characterization and quantification.

Jun 6, 2022 · Blocking the CD28-B7 and CD40-CD40L costimulatory pathways is an effective and synergistic method for the prevention and amelioration of clinical symptoms of experimental autoimmune encephalomyelitis (EAE), a mouse model of MS.

Nov 1, 2023 · Size exclusion chromatography coupled with fluorescence and triple-wavelength ultraviolet detection (SEC-FLD-TWUV) and incorporating an aromatic amino acid of tryptophan as an internal standard offers a simple, rapid, and reliable approach for simultaneous multi-attribute analysis of AAVs.

In this study we take up this question and apply the latest electron microscopy tomographic techniques combined with negative staining to demonstrate the conformational difference between viral capsids that have been filled and those that remain empty.

Mar 19, 2025 · In this study, we sought to restore SCN1A to telencephalic γ-aminobutyric acid (GABA)–producing (GABAergic) interneurons using AAV vectors. To achieve this, we divided the SCN1A ORF into two halves that then underwent intein-mediated ligation to reconstitute a scarless, full-length functional Na V 1.1 after expression. With this split-intein mechanism and using class-specific enhancer DLX2 ...

Mass photometry provides a faster and more straightforward analysis of AAV subspecies by single-molecule counting. It allows the determination of the full-empty ratio (F/E), the molecular weight of empty and filled AAV capsids as well as the mass of the encapsulated genetic material.

Jul 22, 2024 · AAV aggregation analysis by SEC requires the right column to preserve precious samples and achieve sensitive detection. By optimizing sample utilization and implementing efficient short columns, you can streamline fast analyses while maintaining data integrity.