The AAV genome is built of single-stranded deoxyribonucleic acid (ssDNA), either positive- or negative-sensed, which is about 4.7 kilobase long. The genome comprises ITRs at both ends of the DNA strand, and two open reading frames (ORFs): rep and cap.

Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you about AAV components, serotypes, pseudotyping, and more.

Initially distinguished serologically, molecular cloning of AAV genes has identified hundreds of unique AAV strains in numerous species. Its single-stranded genome contains three genes, Rep (Replication), Cap (Capsid), and aap (Assembly).

Apr 3, 2024 · Adeno-associated virus (AAV) has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different...

Mar 20, 2025 · In this study, we used spatial genomics to show that transcriptional crosstalk between individual AAV genomes provides a general method for cell-type-specific expression of large cargo by...

The AAV genome is a single-stranded DNA of approximately 4.7 kb that comprises two alternatively spliced viral genes enclosed within two symmetric inverted terminal repeats (ITRs): rep, required for the four viral replication proteins, and cap, which encodes the three AAV structural capsid proteins . AAV have been widely used in gene therapy as ...

Explore the Adeno-Associated Virus (AAV) genome's structure, functions, and research potential. Discover its unique advantages and challenges. Read more now!

AAV has a linear single-stranded DNA (ssDNA) genome of approximately 4.7-kilobases (kb), with two 145 nucleotide-long inverted terminal repeats (ITR) at the termini. The virus does not encode a polymerase and therefore relies on cellular polymerases for genome replication.

Feb 13, 2018 · Recombinant adeno-associated virus (rAAV)-based gene therapy has entered a phase of clinical translation and commercialization. Despite this progress, vector integrity following production is often overlooked. Compromised vectors …

Mar 14, 2024 · In this study, we present a thorough AAV genome sequencing protocol and analysis pipeline to systematically characterize the molecular state of rAAV genomes at the single-vector level. Leveraging the PacBio SMRT platform, our protocol integrates Bst DNA polymerase and A-tailing DNA end modification.