Production and Characterization of Vectors Based on the …
Vectors based on adeno-associated virus serotype 9 (AAV9) efficiently transduce cardiomyocytes in both rodents and large animal models upon either systemic or regional vector delivery. In …
Alkaline gel measurements of ssDNA from AAV8 vectors Two …
In this study, we present an integrated online native mass spectrometry platform that aims to minimize sample handling and maximize throughput and robustness for rapid and sensitive …
Adeno-Associated Virus-Encapsulated Alginate Microspheres …
AAV-AMs are centrifugally synthesized by ejecting a mixed pre-gel solution of alginate and AAV to CaCl 2 solution to form an ionically cross-linked hydrogel microsphere immediately.
Analysis of Recombinant Adeno-Associated Virus (rAAV) Purity …
Aug 3, 2020 · On a silver-stained SDS-polyacrylamide gel, VP1, VP2, and VP3 should be the only visible bands in a highly purified recombinant adeno-associated virus (rAAV) preparation, …
Quantification of Adeno-Associated Virus with Safe Nucleic Acid …
Here, we report a method to titer AAV with GelGreen ® dye, a safe green fluorescence nucleic acid dye recently engineered by Biotium company (Fremont, CA). This method, hereinafter …
Characterization of Genome Integrity for Oversized Recombinant AAV ...
Nov 10, 2009 · Application of recombinant adeno-associated virus (rAAV) in gene therapy has been limited by its packaging capacity. Recent studies suggested that rAAV could achieve …
Rapid Adeno-Associated Virus Genome Integrity Analysis by Capillary Gel ...
Oct 9, 2020 · In this paper, two adeno-associated virus (AAV) genome integrity analysis workflows are introduced; a standard sample preparation protocol and an accelerated …
Combination of AAV-delivered tumor suppressor PTEN with anti …
Jan 24, 2024 · We demonstrated that intratumorally injected AAV6-PTEN successfully restored the tumor cell PTEN gene expression and effectively inhibited tumor progression by inducing …
Genome integrity analysis of adeno-associated viruses (AAV
Rapid and robust analysis of adeno-associated virus (AAV) genome integrity was achieved by simultaneous separation of multiple samples on a multi-capillary analyzer, the BioPhase 8800.
Adeno-Associated Virus-Encapsulated Alginate Microspheres …
AAV-AMs are centrifugally synthesized by ejecting a mixed pre-gel solution of alginate and AAV to CaCl 2 solution to form an ionically cross-linked hydrogel microsphere immediately.